Proteintech, 66006-2-Ig, HA Tag Monoclonal antibody

Size: 150ul The HA Tag (66006-2-Ig) by Proteintech is a Monoclonal antibody targeting HA Tag in WB, IF/ICC, IP, ELISA applications with reactivity to recombinant protein samples 66006-2-Ig targets HA Tag in WB, IF/ICC, IP, CoIP, ChIP, RIP, ELISA, PLA applications and shows reactivity with recombinant protein samples. Tested Applications Positive WB detected in: Transfected HEK-293 cells, HEK-293 cells, Ag19172 Positive IP detected in: Transfected HEK-293 cells Positive IF/ICC detected in: Transfected HEK-293 cells Recommended dilution Western Blot (WB): WB : 1:10000-1:100000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600 Background Information Protein tags are a protein or peptide sequences located either on the C- or N- terminal of the target protein, which can facilitate solubility, detection, purification, localization, and expression of the target protein. The HA tag corresponds to amino acid residues YPYDVPDYA of a surface glycoprotein -human influenza virus hemagglutinin (HA). The HA tag is commonly used for a variety of research applications including chromatin immunoprecipitation, ELISA, flow cytometry, western blotting, immunocytochemistry/immunofluorescence among of others. Specification Tested Reactivity: recombinant protein Cited Reactivity: human, mouse, rat Host / Isotype: Mouse / IgG1 Class: Monoclonal Type: Antibody Immunogen: Peptide Predict reactive species Full Name: HA Tag Calculated Molecular Weight: 1 kDa RRID: AB_2881490 Conjugate: Unconjugated Form: Liquid Purification Method: Protein G purification UNIPROT ID: HATAG Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.