Proteintech, 66018-1-Ig, TST Monoclonal antibody

Size: 20ul / 150ul The TST (66018-1-Ig) by Proteintech is a Monoclonal antibody targeting TST in WB, IHC, IF/ICC, ELISA applications with reactivity to human, mouse, rat samples 66018-1-Ig targets TST in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: A549 cells, HEK-293 cells, human brain tissue, mouse brain tissue, mouse liver tissue, rat liver tissue Positive IHC detected in: rat kidney tissue, human kidney tissue, human liver cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: A549 cells Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Immunohistochemistry (IHC): IHC : 1:8000-1:32000 Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800 Background Information Thiosulfate sulfurtransferase (TST), also known as rhodanese, is a mitochondrial enzyme involved in cyanide detoxification and the formation of iron-sulfur proteins and the modification of sulfur-containing enzymes. Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human, mouse, rat Host / Isotype: Mouse / IgG2a Class: Monoclonal Type: Antibody Immunogen: CatNo: Ag9235 Product name: Recombinant human TST protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 1-297 aa of BC010148 Sequence: MVHQVLYRALVSTKWLAESIRTGKLGPGLRVLDASWYSPGTREARKEYLERHVPGASFFDIEECRDTASPYEMMLPSEAGFAEYVGRLGISNHTHVVVYDGEHLGSFYAPRVWWMFRVFGHRTVSVLNGGFRNWLKEGHPVTSEPSRPEPAVFKATLDRSLLKTYEQVLENLESKRFQLVDSRSQGRFLGTEPEPDAVGLDSGHIRGAVNMPFMDFLTEDGFEKGPEELRALFQTKKVDLSQPLIATCRKGVTACHVALAAYLCGKPDVAVYDGSWSEWFRRAPPESRVSQGKSEKA Predict reactive species Full Name: thiosulfate sulfurtransferase (rhodanese) Calculated Molecular Weight: 297 aa, 33 kDa Observed Molecular Weight: 33 kDa GenBank Accession Number: BC010148 Gene Symbol: TST Gene ID (NCBI): 7263 RRID: AB_11045660 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: Q16762 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.