Proteintech, 66047-1-Ig, Cyclophilin B Monoclonal antibody

Size: 20ul / 150ul The Cyclophilin B (66047-1-Ig) by Proteintech is a Monoclonal antibody targeting Cyclophilin B in WB, IHC, IF/ICC, FC (Intra), ELISA applications with reactivity to human, mouse samples 66047-1-Ig targets Cyclophilin B in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse samples. Tested Applications Positive WB detected in: HeLa cells, MCF-7 cells, HEK-293 cells, Jurkat cells, RAW 264.7 cells, ROS1728 cells, PC-12 cells, C6 cells Positive IHC detected in: human pancreas cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HeLa cells Positive FC (Intra) detected in: HeLa cells Recommended dilution Western Blot (WB): WB : 1:1000-1:8000 Immunohistochemistry (IHC): IHC : 1:250-1:1000 Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600 Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension Background Information PPIB, also named as CYPB, SCYLP, PPIase B and CYP-S1, belongs to the cyclophilin-type PPIase family and PPIase B subfamily. PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. PPIB is secreted in biological fluids such as blood or milk and binds to a specific receptor present on the human lymphoblastic cell line Jurkat and on human peripheral blood lymphocytes. The full-length of PPIB has 216aa (24 kDa), and a 33aa-signal peptide could be removed in the mature form (about 19 kDa). Specification Tested Reactivity: human, mouse Host / Isotype: Mouse / IgG2a Class: Monoclonal Type: Antibody Immunogen: CatNo: Ag17957 Product name: Recombinant human PPIB protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 1-216 aa of BC032138 Sequence: MLRLSERNMKVLLAAALIAGSVFFLLLPGPSAADEKKKGPKVTVKVYFDLRIGDEDVGRVIFGLFGKTVPKTVDNFVALATGEKGFGYKNSKFHRVIKDFMIQGGDFTRGDGTGGKSIYGERFPDENFKLKHYGPGWVSMANAGKDTNGSQFFITTVKTAWLDGKHVVFGKVLEGMEVVRKVESTKTDSRDKPLKDVIIADCGKIEVEKPFAIAKE Predict reactive species Full Name: peptidylprolyl isomerase B (cyclophilin B) Calculated Molecular Weight: 216 aa, 24 kDa Observed Molecular Weight: 19-24 kDa GenBank Accession Number: BC032138 Gene Symbol: Cyclophilin B Gene ID (NCBI): 5479 RRID: AB_11042327 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: P23284 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.