Proteintech, 66120-1-Ig, Aldolase C Monoclonal antibody

Size: 20ul / 150ul The Aldolase C (66120-1-Ig) by Proteintech is a Monoclonal antibody targeting Aldolase C in WB, IHC, IF/ICC, ELISA applications with reactivity to human, mouse, rat, pig samples 66120-1-Ig targets Aldolase C in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat, pig samples. Tested Applications Positive WB detected in: HeLa cells, human brain tissue, human heart tissue, HepG2 cells, K-562 cells, HSC-T6 cells, Pig brain tissue, Mouse brain tissue, rat cerebellum tissue, mouse cerebellum tissue Positive IHC detected in: human liver cancer tissue, mouse brain tissue, mouse liver tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HepG2 cells Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Immunohistochemistry (IHC): IHC : 1:50-1:500 Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600 Background Information Fructose-bisphosphate aldolase C (ALDOC) reversibly cleaves FBP and F1-P to glyceraldehyde 3-phosphate and dihydroxyacetone phosphate and is strongly expressed in mammalian brain together with ALDOA and is alsopresent in the heart and spleen of some species(PMID:9363598). It is involed in glycolysis as an important enzyme. Phospholipase D2 and inositol 1,4,5-triphosphate interact with ALDOC in signal transduction. Meanwhile, the protein expression of ALDOC has been reported to be regulated in brain tumor, hepatomas, and lung cancer(PMID:21548097). Specification Tested Reactivity: human, mouse, rat, pig Host / Isotype: Mouse / IgG1 Class: Monoclonal Type: Antibody Immunogen: CatNo: Ag6779 Product name: Recombinant human ALDOC protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 1-364 aa of BC003613 Sequence: MPHSYPALSAEQKKELSDIALRIVAPGKGILAADESVGSMAKRLSQIGVENTEENRRLYRQVLFSADDRVKKCIGGVIFFHETLYQKDDNGVPFVRTIQDKGIVVGIKVDKGVVPLAGTDGETTTQGLDGLSERCAQYKKDGADFAKWRCVLKISERTPSALAILENANVLARYASICQQNGIVPIVEPEILPDGDHDLKRCQYVTEKVLAAVYKALSDHHVYLEGTLLKPNMVTPGHACPIKYTPEEIAMATVTALRRTVPPAVPGVTFLSGGQSEEEASFNLNAINRCPLPRPWALTFSYGRALQASALNAWRGQRDNAGAATEEFIKRAEVNGLAAQGKYEGSGEDGGAAAQSLYIANHAY Predict reactive species Full Name: aldolase C, fructose-bisphosphate Calculated Molecular Weight: 39 kDa Observed Molecular Weight: 39 kDa GenBank Accession Number: BC003613 Gene Symbol: Aldolase C Gene ID (NCBI): 230 RRID: AB_2881519 Conjugate: Unconjugated Form: Liquid Purification Method: Protein G purification UNIPROT ID: P09972 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.