Proteintech, 66125-1-Ig, Alpha cardiac muscle actin specific Monoclonal antibody

Size: 20ul / 150ul The Alpha cardiac muscle actin specific (66125-1-Ig) by Proteintech is a Monoclonal antibody targeting Alpha cardiac muscle actin specific in WB, IHC, IF-P, IF-Fro, FC (Intra), ELISA applications with reactivity to human, mouse, rat, pig samples 66125-1-Ig targets Alpha cardiac muscle actin specific in WB, IHC, IF-P, IF-Fro, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat, pig samples. Tested Applications Positive WB detected in: human heart tissue, pig heart tissue, rat heart tissue, mouse heart tissue Positive IHC detected in: human heart tissue, human heart and human skeletal muscle tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF-P detected in: mouse heart tissue Positive IF-Fro detected in: mouse heart tissue Positive FC (Intra) detected in: C2C12 cells Recommended dilution Western Blot (WB): WB : 1:2500-1:10000 Immunohistochemistry (IHC): IHC : 1:100-1:400 Immunofluorescence (IF)-P: IF-P : 1:200-1:800 Immunofluorescence (IF)-FRO: IF-FRO : 1:400-1:1600 Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension Background Information Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. The ACTC1 gene encodes cardiac muscle alpha-actin, the predominant actin isoform in adult heart, which interacts with a variety of proteins to produce the force for muscle contraction. This antibody is specific to the ACTC1. It does not cross-react with other actin isoforms. Specification Tested Reactivity: human, mouse, rat, pig Cited Reactivity: human, mouse, rat, rabbit Host / Isotype: Mouse / IgG1 Class: Monoclonal Type: Antibody Immunogen: Peptide Predict reactive species Full Name: actin, alpha, cardiac muscle 1 Calculated Molecular Weight: 42 kDa Observed Molecular Weight: 42 kDa GenBank Accession Number: NM_005159 Gene Symbol: ACTC1 Gene ID (NCBI): 70 RRID: AB_2881524 Conjugate: Unconjugated Form: Liquid Purification Method: Protein G purification UNIPROT ID: P68032 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.