Proteintech, 66280-1-Ig, Gelsolin Monoclonal antibody

Size: 20ul / 150ul The Gelsolin (66280-1-Ig) by Proteintech is a Monoclonal antibody targeting Gelsolin in WB, IHC, ELISA applications with reactivity to human samples 66280-1-Ig targets Gelsolin in WB, IHC, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: human plasma tissue, U2OS cells, A549 cells, human plasma, HT-29 cells Positive IHC detected in: human colon tissue, human colon cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:1000-1:4000 Immunohistochemistry (IHC): IHC : 1:20-1:1000 Background Information Gelsolin (GSN) is an actin binding protein that regulates actin-severing/depolymerizing in a calcium dependent manner, and acts as a multifunctional regulator for cell metabolism and survival. Gelsolin exists in two isoforms: a cytoplasmic gelsolin (cGSN; 80-90 kDa) and a plasma gelsolin (pGSN; 93 kDa), differing at N terminal residues. Decreased levels of pGSN are strongly associated with human diseases, such as, acute liver injury, major trauma, myocardial infarction, inflammation, lung injury, rheumatoid arthritis, hemodialysis, multiple sclerosis, Alzheimer's disease. Specification Tested Reactivity: human Host / Isotype: Mouse / IgA Class: Monoclonal Type: Antibody Immunogen: CatNo: Ag17916 Product name: Recombinant human Gelsolin protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 433-782 aa of BC026033 Sequence: MAAQHGMDDDGTGQKQIWRIEGSNKVPVDPATYGQFYGGDSYIILYNYRHGGRQGQIIYNWQGAQSTQDEVAASAILTAQLDEELGGTPVQSRVVQGKEPAHLMSLFGGKPMIIYKGGTSREGGQTAPASTRLFQVRANSAGATRAVEVLPKAGALNSNDAFVLKTPSAAYLWVGTGASEAEKTGAQELLRVLRAQPVQVAEGSEPDGFWEALGGKAAYRTSPRLKDKKMDAHPPRLFACSNKIGRFVIEEVPGELMQEDLATDDVMLLDTWDQVFVWVGKDSQEEEKTEALTSAKRYIETDPANRDRRTPITVVKQGFEPPSFVGWFLGWDDDYWSVDPLDRAMAELAA Predict reactive species Full Name: gelsolin (amyloidosis, Finnish type) Calculated Molecular Weight: 782 aa, 86 kDa Observed Molecular Weight: 80-83 kDa GenBank Accession Number: BC026033 Gene Symbol: Gelsolin Gene ID (NCBI): 2934 RRID: AB_2881663 Conjugate: Unconjugated Form: Liquid Purification Method: Thiophilic affinity chromatograph UNIPROT ID: P06396 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.