Proteintech, 66367-1-Ig, SKAP2 Monoclonal antibody

Size: 20ul / 150ul The SKAP2 (66367-1-Ig) by Proteintech is a Monoclonal antibody targeting SKAP2 in WB, IHC, IF/ICC, ELISA applications with reactivity to human, pig, rat, mouse samples 66367-1-Ig targets SKAP2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, pig, rat, mouse samples. Tested Applications Positive WB detected in: RAW 264.7 cells, human spleen tissue, pig liver tissue Positive IHC detected in: human colon cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: NIH/3T3 cells Recommended dilution Western Blot (WB): WB : 1:500-1:2000 Immunohistochemistry (IHC): IHC : 1:50-1:500 Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600 Background Information SKAP2 (src kinase associated phosphoprotein 2), also named PRAP, contains PH domain and SH3 domain. It is localized in cytoplasm and was expressed in ubiquitously in various tissues including lung, skeletal muscle, and spleen, but not detectable in thymus and T cell lymphoma cells. SKAP2 may negatively regulate alpha-synuclein phosphorylation following cell stress. SKAP2 may be involved in B-cell and macrophage adhesion processes by coupling the B-cell receptor (BCR) to integrin activation. Specification Tested Reactivity: human, pig, rat, mouse Host / Isotype: Mouse / IgG1 Class: Monoclonal Type: Antibody Immunogen: CatNo: Ag3615 Product name: Recombinant human SKAP2 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-359 aa of BC036044 Sequence: MPNPSSTSSPYPLPEEIRNLLADVETFVADILKGENLSKKAKEKRESLIKKIKDVKSIYLQEFQDKGDAEDGEEYDDPFAGPPDTISLASERYDKDDEAPSDGAQFPPIAAQDLPFVLKAGYLEKRRKDHSFLGFEWQKRWCALSKTVFYYYGSDKDKQQKGEFAIDGYSVRMNNTLRKDGKKDCCFEISAPDKRIYQFTAASPKDAEEWVQQLKFVLQDMESDIIPEDYDERGELYDDVDHPLPISNPLTSSQPIDDEIYEELPEEEEDSAPVKVEEQRKMSQDSVHHTSGDKSTDYANFYQGLWDCTGAFSDELSFKRGDVIYILSKEYNRYGWWVGEMKGAIGLVPKAYIMEMYDI Predict reactive species Full Name: src kinase associated phosphoprotein 2 Calculated Molecular Weight: 41 kDa Observed Molecular Weight: 55 kDa GenBank Accession Number: BC036044 Gene Symbol: SKAP2 Gene ID (NCBI): 8935 RRID: AB_2881747 Conjugate: Unconjugated Form: Liquid Purification Method: Protein G purification UNIPROT ID: O75563 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.