Proteintech, 66408-1-Ig, Human IgG4 Monoclonal antibody

Size: 20ul / 150ul The Human IgG4 (66408-1-Ig) by Proteintech is a Monoclonal antibody targeting Human IgG4 in WB, IHC, IF-P, ELISA applications with reactivity to human samples 66408-1-Ig targets Human IgG4 in WB, IHC, IF-P, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: human plasma tissue, human heart tissue, human skeletal muscle tissue Positive IHC detected in: human tonsillitis tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF-P detected in: human appendicitis tissue Recommended dilution Western Blot (WB): WB : 1:20000-1:100000 Immunohistochemistry (IHC): IHC : 1:2500-1:10000 Immunofluorescence (IF)-P: IF-P : 1:200-1:800 Background Information This antibody detects the heavy chain of human IgG4. The immunogen of this antibody is the specific domain "NKGLPSSIEK" of IGHG4. Specification Tested Reactivity: human Host / Isotype: Mouse / IgG1 Class: Monoclonal Type: Antibody Immunogen: Peptide Predict reactive species Full Name: immunoglobulin heavy constant gamma 4 (G4m marker) Calculated Molecular Weight: 55 kDa Observed Molecular Weight: 55 kDa GenBank Accession Number: AL928742 Gene Symbol: IGHG4 Gene ID (NCBI): 3503 RRID: AB_2881780 Conjugate: Unconjugated Form: Liquid Purification Method: Protein G purification Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.