Proteintech, 66422-1-Ig, PDI Monoclonal antibody

Size: 20ul / 150ul The PDI (66422-1-Ig) by Proteintech is a Monoclonal antibody targeting PDI in WB, IHC, IF/ICC, ELISA applications with reactivity to human, mouse, rat, pig samples 66422-1-Ig targets PDI in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat, pig samples. Tested Applications Positive WB detected in: HEK-293 cells, rat brain tissue, HepG2 cells, L02 cells, COLO 320 cells Positive IHC detected in: human liver tissue, human small intestine tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HepG2 cells Recommended dilution Western Blot (WB): WB : 1:10000-1:100000 Immunohistochemistry (IHC): IHC : 1:100-1:400 Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800 Background Information PDIA1(Protein disulfide-isomerase) is also named as ERBA2L, PDI, P4HB, PO4DB. It is a multifunctional protein that catalyzes the formation, breakage and rearrangement of disulfide bonds. In some cell types, it seems to be secreted or associated with the plasma membrane, where it undergoes constant shedding and replacement from intracellular sources.It can exsit as homodimer and monomers and homotetramers may also occur(PMID:12095988). Specification Tested Reactivity: human, mouse, rat, pig Cited Reactivity: human, mouse, bovine Host / Isotype: Mouse / IgG2b Class: Monoclonal Type: Antibody Immunogen: CatNo: Ag1747 Product name: Recombinant human PDI protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 89-273 aa of BC014504 Sequence: MTKYKPESEELTAEWITEFCHRFLEGKIKPHLMSQELPEDWDKQPVKVLVGKNFEDVAFDEKKNVFVEFYAPWCGHCKQLAPIWDKLGETYKDHENIVIAKMDSTANEVEAVKVHSFPTLKFFPASADRTVIDYNGERTLDGFKKFLESGGQDGAGDDDDLEDLEEAEEPDMEEDDDQKAVKDEL Predict reactive species Full Name: prolyl 4-hydroxylase, beta polypeptide Calculated Molecular Weight: 57 kDa Observed Molecular Weight: 57 kDa GenBank Accession Number: BC014504 Gene Symbol: PDI Gene ID (NCBI): 5034 RRID: AB_2881794 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: P07237 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.