Proteintech, 66423-1-Ig, ERp57/ERp60 Monoclonal antibody

Size: 20ul / 150ul The ERp57/ERp60 (66423-1-Ig) by Proteintech is a Monoclonal antibody targeting ERp57/ERp60 in WB, IHC, IF/ICC, ELISA applications with reactivity to human, mouse, rat samples 66423-1-Ig targets ERp57/ERp60 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: U2OS cells, HeLa cells, HEK-293 cells, Jurkat cells, RAW 264.7 cells, LNCaP cells, K-562 cells, HSC-T6 cells, PC-12 cells, NIH/3T3 cells Positive IHC detected in: human lung cancer tissue, human liver cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HepG2 cells Recommended dilution Western Blot (WB): WB : 1:2000-1:20000 Immunohistochemistry (IHC): IHC : 1:200-1:800 Immunofluorescence (IF)/ICC: IF/ICC : 1:500-1:2000 Background Information PDIA3, also named as P58, ER60, ERp57, ERp60, ERp61, GRP57, GRP58 and PI-PLC, is a member of the PDI family, participates in the oxidation, reduction, and isomerization of disulfide bonds for correct folding of secretory proteins before modification and transport in the endoplasmic reticulum. It is associated with apoptosis or inhibition of cancer cell growth. PDIA3 was once thought to be a phospholipase; however, it has been demonstrated that this protein actually has protein disulfide isomerase activity. It is thought that complexes of lectins and PDIA3 mediate protein folding by promoting formation of disulfide bonds in their glycoprotein substrates. Specification Tested Reactivity: human, mouse, rat Host / Isotype: Mouse / IgG1 Class: Monoclonal Type: Antibody Immunogen: CatNo: Ag8741 Product name: Recombinant human PDIA3 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 161-505 aa of BC014433 Sequence: DDSFSEAHSEFLKAASNLRDNYRFAHTNVESLVNEYDDNGEGIILFRPSHLTNKFEDKTVAYTEQKMTSGKIKKFIQENIFGICPHMTEDNKDLIQGKDLLIAYYDVDYEKNAKGSNYWRNRVMMVAKKFLDAGHKLNFAVASRKTFSHELSDFGLESTAGEIPVVAIRTAKGEKFVMQEEFSRDGKALERFLQDYFDGNLKRYLKSEPIPESNDGPVKVVVAENFDEIVNNENKDVLIEFYAPWCGHCKNLEPKYKELGEKLSKDPNIVIAKMDATANDVPSPYEVRGFPTIYFSPANKKLNPKKYEGGRELSDFISYLQREATNPPVIQEEKPKKKKKAQEDL Predict reactive species Full Name: protein disulfide isomerase family A, member 3 Calculated Molecular Weight: 505 aa, 57 kDa Observed Molecular Weight: 57 kDa, 60 kDa GenBank Accession Number: BC014433 Gene Symbol: PDIA3 Gene ID (NCBI): 2923 RRID: AB_2881795 Conjugate: Unconjugated Form: Liquid Purification Method: Protein G purification UNIPROT ID: P30101 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.