Proteintech, 66431-1-Ig, PLEK Monoclonal antibody

Size: 20ul / 150ul The PLEK (66431-1-Ig) by Proteintech is a Monoclonal antibody targeting PLEK in WB, IHC, IF-P, ELISA applications with reactivity to human, pig, mouse samples 66431-1-Ig targets PLEK in WB, IHC, IF-P, ELISA applications and shows reactivity with human, pig, mouse samples. Tested Applications Positive WB detected in: HL-60 cells, pig spleen tissue, THP-1 cells, U-937 cells Positive IHC detected in: human colon cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF-P detected in: mouse spleen tissue Recommended dilution Western Blot (WB): WB : 1:2000-1:20000 Immunohistochemistry (IHC): IHC : 1:50-1:500 Immunofluorescence (IF)-P: IF-P : 1:200-1:800 Background Information PLEK (pleckstrin; also known as p47) was originally identified as the major PKC substrate in platelets and later is found to be expressed in all cells of the hemopoietic system. Through two pleckstrin homology (PH) domains at its N and C termini, PLEK may interact with various protein and/or lipid ligands and serve as an intracellular adaptor/targeting protein. Predominantly cytosolic in unstimulated cells, PLEK would undergo transient redistribution to phagosomal membrane in response to stimuli. PLEK has been found to be hyperphosphorylated in diabetic mononuclear phagocytes and promote proinflammatory cytokine secretion in diabetes. (10477609, 17579087) Specification Tested Reactivity: human, pig, mouse Cited Reactivity: human Host / Isotype: Mouse / IgG1 Class: Monoclonal Type: Antibody Immunogen: CatNo: Ag3187 Product name: Recombinant human PLEK protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-350 aa of BC018549 Sequence: MEPKRIREGYLVKKGSVFNTWKPMWVVLLEDGIEFYKKKSDNSPKGMIPLKGSTLTSPCQDFGKRMFVFKITTTKQQDHFFQAAFLEERDAWVRDIKKAIKCIEGGQKFARKSTRRSIRLPETIDLGALYLSMKDTEKGIKELNLEKDKKIFNHCFTGNCVIDWLVSNQSVRNRQEGLMIASSLLNEGYLQPAGDMSKSAVDGTAENPFLDNPDAFYYFPDSGFFCEENSSDDDVILKEEFRGVIIKQGCLLKQGHRRKNWKVRKFILREDPAYLHYYDPAGAEDPLGAIHLRGCVVTSVESNSNGRKSEEENLFEIITADEVHYFLQAATPKERTEWIKAIQMASRTGK Predict reactive species Full Name: pleckstrin Calculated Molecular Weight: 350 aa, 40 kDa Observed Molecular Weight: 40-47 kDa GenBank Accession Number: BC018549 Gene Symbol: PLEK Gene ID (NCBI): 5341 RRID: AB_2881802 Conjugate: Unconjugated Form: Liquid Purification Method: Protein G purification UNIPROT ID: P08567 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.