Proteintech, 66479-1-Ig, Hemopexin Monoclonal antibody

Size: 20ul / 150ul The Hemopexin (66479-1-Ig) by Proteintech is a Monoclonal antibody targeting Hemopexin in WB, IHC, IF-P, FC (Intra), ELISA applications with reactivity to human, rat, pig samples 66479-1-Ig targets Hemopexin in WB, IHC, IF-P, FC (Intra), ELISA applications and shows reactivity with human, rat, pig samples. Tested Applications Positive WB detected in: human plasma tissue, human placenta tissue, rat serum tissue, pig plasma tissue Positive IHC detected in: human liver tissue, human placenta tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF-P detected in: human liver cancer tissue Positive FC (Intra) detected in: HepG2 cells Recommended dilution Western Blot (WB): WB : 1:5000-1:40000 Immunohistochemistry (IHC): IHC : 1:250-1:1000 Immunofluorescence (IF)-P: IF-P : 1:200-1:800 Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.50 ug per 10^6 cells in a 100 µl suspension Background Information Hemopexin (HPX) is the plasma protein responsible for scavenging heme, thus preventing heme-mediated oxidative stress and heme-bound iron loss. In addition, hemopexin blocks heme activation of immune receptors and vascular inflammatory processes. It is mainly expressed in liver, the synthesis of which is induced after inflammation. Alterations of plasma hemopexin level have been linked to disorders like atherosclerosis and inflammatory diseases. Specification Tested Reactivity: human, rat, pig Cited Reactivity: human Host / Isotype: Mouse / IgG1 Class: Monoclonal Type: Antibody Immunogen: CatNo: Ag8533 Product name: Recombinant human HPX protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 45-254 aa of BC005395 Sequence: DVTERCSDGWSFDATTLDDNGTMLFFKGEFVWKSHKWDRELISERWKNFPSPVDAAFRQGHNSVFLIKGDKVWVYPPEKKEKGYPKLLQDEFPGIPSPLDAAVECHRGECQAEGVLFFQGDREWFWDLATGTMKERSWPAVGNCSSALRWLGRYYCFQGNQFLRFDPVRGEVPPRYPRDVRDYFMPCPGRGHGHRNGTGHGNSICAVAHI Predict reactive species Full Name: hemopexin Calculated Molecular Weight: 254 aa, 29 kDa Observed Molecular Weight: 65-75 kDa GenBank Accession Number: BC005395 Gene Symbol: Hemopexin Gene ID (NCBI): 3263 RRID: AB_2881845 Conjugate: Unconjugated Form: Liquid Purification Method: Protein G purification UNIPROT ID: P02790 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.