Proteintech, 66495-1-Ig, SURVIVIN Monoclonal antibody

Size: 20ul / 150ul The SURVIVIN (66495-1-Ig) by Proteintech is a Monoclonal antibody targeting SURVIVIN in WB, IHC, ELISA applications with reactivity to human samples 66495-1-Ig targets SURVIVIN in WB, IHC, IF, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: HEK-293 cells, Raji cells, K-562 cells, HeLa cells, A431 cells, U-937 cells, Jurkat cells Positive IHC detected in: human tonsillitis tissue, human skin cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:1000-1:6000 Immunohistochemistry (IHC): IHC : 1:500-1:1000 Background Information Survivin, also called BIRC5, is a unique member of the inhibitor of apoptosis (IAP) protein family. Survivin is a 16 kDa anti-apoptotic protein highly expressed during fetal development and cancer cell malignancy, but is completely absent in terminally differentiated cells. The differential expression of survivin in cancer versus normal tissues makes it a useful tool in cancer diagnosis and a promising therapeutic target. Survivin expression is also highly regulated by the cell cycle and is only expressed in the G2-M phase. It is known that survivin localizes to the mitotic spindle by interaction with tubulin during mitosis and may play a contributing role in regulating mitosis. Disruption of survivin-microtubule interactions results in loss of survivin's anti-apoptosis function and increased caspase-3 activity, a mechanism involved in cell death, during mitosis. It also is a direct target gene of the Wnt pathway and is upregulated by beta-catenin. Specification Tested Reactivity: human Cited Reactivity: human, mouse, rat Host / Isotype: Mouse / IgG1 Class: Monoclonal Type: Antibody Immunogen: CatNo: Ag20958 Product name: Recombinant human SURVIVIN protein Source: e coli. -derived, PET30a Tag: 6*His Domain: 1-142 aa of BC008718 Sequence: MGAPTLPPAWQPFLKDHRISTFKNWPFLEGCACTPERMAEAGFIHCPTENEPDLAQCFFCFKELEGWEPDDDPIEEHKKHSSGCAFLSVKKQFEELTLGEFLKLDRERAKNKIAKETNNKKKEFEETAKKVRRAIEQLAAMD Predict reactive species Full Name: baculoviral IAP repeat-containing 5 Calculated Molecular Weight: 16 kDa Observed Molecular Weight: 18 kDa GenBank Accession Number: BC008718 Gene Symbol: Survivin Gene ID (NCBI): 332 RRID: AB_2881860 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: O15392 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.