Proteintech, 66515-1-Ig, E2F1 Monoclonal antibody

Size: 20ul / 150ul The E2F1 (66515-1-Ig) by Proteintech is a Monoclonal antibody targeting E2F1 in WB, ELISA applications with reactivity to human, rat samples 66515-1-Ig targets E2F1 in WB, IHC, IF, IP, CoIP, ChIP, ELISA applications and shows reactivity with human, rat samples. Tested Applications Positive WB detected in: LNCaP cells, HepG2 cells, HeLa cells, HEK-293 cells, Jurkat cells, HSC-T6 cells Recommended dilution Western Blot (WB): WB : 1:1000-1:6000 Background Information Transcription factor E2F1 (E2F1), also known as RBBP3, is a transcription activator that binds DNA cooperatively with dp proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from G1 to S phase. E2F-1 binds preferentially RB1 protein, in a cell-cycle dependent manner. It can mediate both cell proliferation and p53-dependent apoptosis. The calculated molecular weight of E2F1 is 47 kDa, but the sumoylated E2F1 is bout 55-60 kDa. Specification Tested Reactivity: human, rat Cited Reactivity: human, mouse, rat Host / Isotype: Mouse / IgG2b Class: Monoclonal Type: Antibody Immunogen: CatNo: Ag17363 Product name: Recombinant human E2F1 protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 88-438 aa of BC050369 Sequence: VKRRLDLETDHQYLAESSGPARGRGRHPGKGVKSPGEKSRYETSLNLTTKRFLELLSHSADGVVDLNWAAEVLKVQKRRIYDITNVLEGIQLIAKKSKNHIQWLGSHTTVGVGGRLEGLTQDLRQLQESEQQLDHLMNICTTQLRLLSEDTDSQRLAYVTCQDLRSIADPAEQMVMVIKAPPETQLQAVDSSENFQISLKSKQGPIDVFLCPEETVGGISPGKTPSQEVTSEEENRATDSATIVSPPPSSPPSSLTTDPSQSLLSLEQEPLLSRMGSLRAPVDEDRLSPLVAADSLLEHVREDFSGLLPEEFISLSPPHEALDYHFGLEEGEGIRDLFDCDFGDLTPLDF Predict reactive species Full Name: E2F transcription factor 1 Calculated Molecular Weight: 437 aa, 47 kDa Observed Molecular Weight: 55-60 kDa GenBank Accession Number: BC050369 Gene Symbol: E2F1 Gene ID (NCBI): 1869 RRID: AB_2881878 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: Q01094 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.