Proteintech, 66618-2-Ig, SPI1 Monoclonal antibody

Size: 20ul / 150ul The SPI1 (66618-2-Ig) by Proteintech is a Monoclonal antibody targeting SPI1 in WB, IHC, ELISA applications with reactivity to human, mouse, rat samples 66618-2-Ig targets SPI1 in WB, IHC, IF, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: Daudi cells, Raji cells, RAW 264.7 cells, rat spleen tissue Positive IHC detected in: Human tonsillitis tissue, mouse spleen tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:2000-1:10000 Immunohistochemistry (IHC): IHC : 1:2000-1:8000 Background Information SPI1, also named as 31 kDa-transforming protein, is a 270 amino acid protein, which belongs to the ETS family. SPI1 is highly expressed in both FV-P and FV-A-induced erythro-leukemia cell lines that have undergone rearrangements of the SPI1 gene due to the insertion of SFFV. Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human, mouse Host / Isotype: Mouse / IgG1 Class: Monoclonal Type: Antibody Immunogen: Peptide Predict reactive species Full Name: spleen focus forming virus (SFFV) proviral integration oncogene spi1 Calculated Molecular Weight: 31 kDa Observed Molecular Weight: 32-38 kDa GenBank Accession Number: NM_003120 Gene Symbol: SPI1 Gene ID (NCBI): 6688 ENSEMBL Gene ID: ENSG00000066336 RRID: AB_2881978 Conjugate: Unconjugated Form: Liquid Purification Method: Protein G purification UNIPROT ID: P17947 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.