Proteintech, 66656-1-Ig, WNT2 Monoclonal antibody

Size: 20ul / 150ul The WNT2 (66656-1-Ig) by Proteintech is a Monoclonal antibody targeting WNT2 in WB, IF/ICC, ELISA applications with reactivity to human, mouse, rat samples 66656-1-Ig targets WNT2 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: HeLa cells, HSC-T6 cells, Jurkat cells, RAW 264.7 cells, rat brain tissue, NIH/3T3 cells, mouse brain tissue, HEK-293 cells, MCF-7 cells Positive IF/ICC detected in: MCF-7 cells Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500 Background Information Wnt2 is a secreted glycoprotein that is one of the canonical Wnt ligands. The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. Wnt2 is known to directly regulate β-catenin activity, the key player in proliferation and inflammation. Recently, studies have demonstrated that Wnt2 is overexpressed in various human cancers, including esophageal, gastric, colorectal, breast, lung, cervical, and malignant glioma. Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human, mouse, rat Host / Isotype: Mouse / IgG1 Class: Monoclonal Type: Antibody Immunogen: CatNo: Ag25185 Product name: Recombinant human WNT2 protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 1-360 aa of BC029854 Sequence: MNAPLGGIWLWLPLLLTWLTPEVNSSWWYMRATGGSSRVMCDNVPGLVSSQRQLCHRHPDVMRAISQGVAEWTAECQHQFRQHRWNCNTLDRDHSLFGRVLLRSSRESAFVYAISSAGVVFAITRACSQGEVKSCSCDPKKMGSAKDSKGIFDWGGCSDNIDYGIKFARAFVDAKERKGKDARALMNLHNNRAGRKAVKRFLKQECKCHGVSGSCTLRTCWLAMADFRKTGDYLWRKYNGAIQVVMNQDGTGFTVANERFKKPTKNDLVYFENSPDYCIRDREAGSLGTAGRVCNLTSRGMDSCEVMCCGRGYDTSHVTRMTKCGCKFHWCCAVRCQDCLEALDVHTCKAPKNADWTTAT Predict reactive species Full Name: wingless-type MMTV integration site family member 2 Calculated Molecular Weight: 40 kDa Observed Molecular Weight: 36 kDa GenBank Accession Number: BC029854 Gene Symbol: WNT2 Gene ID (NCBI): 7472 RRID: AB_2882013 Conjugate: Unconjugated Form: Liquid Purification Method: Protein G purification UNIPROT ID: P09544 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.