Proteintech, 66744-1-Ig, ATG5 Monoclonal antibody

Size: 20ul / 150ul The ATG5 (66744-1-Ig) by Proteintech is a Monoclonal antibody targeting ATG5 in WB, IHC, ELISA applications with reactivity to human, mouse, rat samples 66744-1-Ig targets ATG5 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: HEK-293 cells, HepG2 cells, HeLa cells, Jurkat cells, Raji cells, HSC-T6 cells, NIH/3T3 cells Positive IHC detected in: human colon cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:1000-1:6000 Immunohistochemistry (IHC): IHC : 1:150-1:600 Background Information ATG5, also named as APG5L and ASP, belongs to the ATG5 family. It is required for autophagy. It plays an important role in the apoptotic process, possibly within the modified cytoskeleton. Its expression is a relatively late event in the apoptotic process, occurring downstream of caspase activity. Autophagy is a catabolic process for the autophagosomic-lysosomal degradation of bulk cytoplasmic contents. Formation of the autophagosome involves a ubiquitin-like conjugation system in which ATG12 is covalently bound to ATG5 and targeted to autophagosome vesicles. It mediates autophagosome-independent host protection. 66744-1-Ig antibody can recognize the ATG5-ATG12 complex (50-55 kDa) which can be truncated and generate a 40-45 kDa band. (PMID: 19783656, 25032862) Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human, mouse, rat, pig Host / Isotype: Mouse / IgG2a Class: Monoclonal Type: Antibody Immunogen: CatNo: Ag16685 Product name: Recombinant human ATG5 protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 28-275 aa of BC002699 Sequence: TEREAEPYYLLLPRVSYLTLVTDKVKKHFQKVMRQEDISEIWFEYEGTPLKWHYPIGLLFDLLASSSALPWNITVHFKSFPEKDLLHCPSKDAIEAHFMSCMKEADALKHKSQVINEMQKKDHKQLWMGLQNDRFDQFWAINRKLMEYPAEENGFRYIPFRIYQTTTERPFIQKLFRPVAADGQLHTLGDLLKEVCPSAIDPEDGEKKNQVMIHGIEPMLETPLQWLSEHLSYPDNFLHISIIPQPTD Predict reactive species Full Name: ATG5 autophagy related 5 homolog (S. cerevisiae) Calculated Molecular Weight: 32 kDa Observed Molecular Weight: 50-55 and 40-45 kDa GenBank Accession Number: BC002699 Gene Symbol: ATG5 Gene ID (NCBI): 9474 RRID: AB_2882092 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: Q9H1Y0 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.