Proteintech, 66753-1-Ig, Factor X Monoclonal antibody

Size: 20ul / 150ul The Factor X (66753-1-Ig) by Proteintech is a Monoclonal antibody targeting Factor X in WB, IHC, IF-P, ELISA applications with reactivity to human samples 66753-1-Ig targets Factor X in WB, IHC, IF-P, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: human plasma tissue Positive IHC detected in: human liver tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF-P detected in: human liver tissue Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Immunohistochemistry (IHC): IHC : 1:500-1:2000 Immunofluorescence (IF)-P: IF-P : 1:200-1:800 Background Information Coagulation factor X (F10) is a vitamin K-dependent glycoprotein that participates in the middle phase of the blood coagulation cascade (PMID: 1931959; 1313796). It is synthesized as a single-chain precursor in the liver where it undergoes a number of covalent processing steps before secretion into the blood as a two-chain molecule linked by a disulfide bond (PMID: 1313796). The light chain contains 2 EGF-like domains, while the heavy chain contains the catalytic domain which is structurally homologous to those of the other hemostatic serine proteases. Factor X is activated into factor Xa, by both the extrinsic and intrinsic pathway. The activated factor then converts prothrombin to thrombin in the presence of factor Va, calcium and phospholipid during blood clotting. Specification Tested Reactivity: human Host / Isotype: Mouse / IgG1 Class: Monoclonal Type: Antibody Immunogen: CatNo: Ag11664 Product name: Recombinant human F10 protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 140-488 aa of BC046125 Sequence: CHEEQNSVVCSCARGYTLADNGKACIPTGPYPCGKQTLERRKRSVAQATSSSGEAPDSITWKPYDAADLDPTENPFDLLDFNQTQPERGDNNLTRIVGGQECKDGECPWQALLINEENEGFCGGTILSEFYILTAAHCLYQAKRFKVRVGDRNTEQEEGGEAVHEVEVVIKHNRFTKETYDFDIAVLRLKTPITFRMNVAPACLPERDWAESTLMTQKTGIVSGFGRTHEKGRQSTRLKMLEVPYVDRNSCKLSSSFIITQNMFCAGYDTKQEDACQGDSGGPHVTRFKDTYFVTGIVSWGEGCARKGKYGIYTKVTAFLKWIDRSMKTRGLPKAKSHAPEVITSSPLK Predict reactive species Full Name: coagulation factor X Calculated Molecular Weight: 488 aa, 55 kDa Observed Molecular Weight: 50 kDa GenBank Accession Number: BC046125 Gene Symbol: F10 Gene ID (NCBI): 2159 RRID: AB_2882099 Conjugate: Unconjugated Form: Liquid Purification Method: Protein G purification UNIPROT ID: P00742 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.