Proteintech, 66757-1-Ig, AURKA Monoclonal antibody

Size: 20ul / 150ul The AURKA (66757-1-Ig) by Proteintech is a Monoclonal antibody targeting AURKA in WB, IHC, IF/ICC, ELISA applications with reactivity to human, mouse, rat samples 66757-1-Ig targets AURKA in WB, IHC, IF/ICC, CoIP, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: human testis tissue, HeLa cells, NCCIT cells, rat testis tissue, mouse testis tissue Positive IHC detected in: human breast cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HeLa cells, A431 cells Recommended dilution Western Blot (WB): WB : 1:1000-1:6000 Immunohistochemistry (IHC): IHC : 1:300-1:1200 Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600 Background Information AURKA(Aurora kinase A), also named as STK6, STK15 or AIK, belongs to the Ser/Thr protein kinase family. This protein may play a role in cell cycle regulation during anaphase and/or telophase by being involved in microtubule formation and/or stabilization. Some recent evidence revealed that AURKA may also be implicated in tumor development and progression. AURKA is expressed highly in testis and various proliferating cells, migrating as a 46 kDa protein in SDS PAGE.Nuclear staining for AURKA is weak or nonexistent in normal tissue but strong in tumor tissue(PMID:19107951). Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human, mouse Host / Isotype: Mouse / IgG3 Class: Monoclonal Type: Antibody Immunogen: CatNo: Ag20096 Product name: Recombinant human AURKA protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 108-337 aa of BC002499 Sequence: NNPEEELASKQKNEESKKRQWALEDFEIGRPLGKGKFGNVYLAREKQSKFILALKVLFKAQLEKAGVEHQLRREVEIQSHLRHPNILRLYGYFHDATRVYLILEYAPLGTVYRELQKLSKFDEQRTATYITELANALSYCHSKRVIHRDIKPENLLLGSAGELKIADFGWSVHAPSSRRTTLCGTLDYLPPEMIEGRMHDEKVDLWSLGVLCYEFLVGKPPFEANTYQET Predict reactive species Full Name: aurora kinase A Calculated Molecular Weight: 46 kDa Observed Molecular Weight: 46-52 kDa GenBank Accession Number: BC002499 Gene Symbol: AURKA Gene ID (NCBI): 6790 RRID: AB_2882103 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: O14965 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.