Proteintech, 66775-1-Ig, Syntaxin 16 Monoclonal antibody

Size: 20ul / 150ul The Syntaxin 16 (66775-1-Ig) by Proteintech is a Monoclonal antibody targeting Syntaxin 16 in WB, IHC, IF/ICC, ELISA applications with reactivity to Human, Mouse, Rat samples 66775-1-Ig targets Syntaxin 16 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, Mouse, Rat samples. Tested Applications Positive WB detected in: HeLa cells, rat brain tissue, mouse brain tissue, HEK-293 cells, HepG2 cells, Jurkat cells Positive IHC detected in: human lung cancer tissue, human colon cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: Neuro-2a cells Recommended dilution Western Blot (WB): WB : 1:2000-1:10000 Immunohistochemistry (IHC): IHC : 1:250-1:1000 Immunofluorescence (IF)/ICC: IF/ICC : 1:100-1:800 Background Information Syntaxin 16 (STX16), also named as SYN16, belongs to the syntaxin family. Syntaxins are target membrane SNAREs(Soluble NSF attachment protein receptors) which are membrane components that determine the specificity of the docking and fusion of vesicles to target membranes. STX16 is localized to the Golgi apparatus. Specification Tested Reactivity: Human, Mouse, Rat Host / Isotype: Mouse / IgG1 Class: Monoclonal Type: Antibody Immunogen: CatNo: Ag27926 Product name: Recombinant human STX16 protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 1-304 aa of BC019042 Sequence: MATRRLTDAFLLLRNNSIQNRQLLAEQLADDRMALVSGISLDPEAAIGVTKRPPPKWVDGVDEIQYDVGRIKQKMKELASLHDKHLNRPTLDDSSEEEHAIEITTQEITQLFHRCQRAVQALPSRARACSEQEGRLLGNVVASLAQALQELSTSFRHAQSGYLKRMKNREERSQHFFDTSVPLMDDGDDNTLYHRGFTEDQLVLVEQNTLMVEEREREIRQIVQSISDLNEIFRDLGAMIVEQGTVLDRIDYNVEQSCIKTEDGLKQLHKAEQYQKKNRKMLVILILFVIIIVLIVVLVGVKSR Predict reactive species Full Name: syntaxin 16 Calculated Molecular Weight: 37 kDa Observed Molecular Weight: 37-40 kDa GenBank Accession Number: BC019042 Gene Symbol: Syntaxin 16 Gene ID (NCBI): 8675 RRID: AB_2882121 Conjugate: Unconjugated Form: Liquid Purification Method: Protein G purification UNIPROT ID: O14662 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.