Proteintech, 66806-1-Ig, EEF1A1/EEF1A2 Monoclonal antibody

Size: 20ul / 150ul The EEF1A1/EEF1A2 (66806-1-Ig) by Proteintech is a Monoclonal antibody targeting EEF1A1/EEF1A2 in WB, IHC, ELISA applications with reactivity to human, mouse, rat, pig samples 66806-1-Ig targets EEF1A1/EEF1A2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat, pig samples. Tested Applications Positive WB detected in: HeLa cells, MCF-7 cells, mouse brain tissue, pig brain tissue, rat brain tissue, HEK-293 cells, Jurkat cells, HSC-T6 cells, NIH/3T3 cells, 4T1 cells Positive IHC detected in: rat brain tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:1000-1:6000 Immunohistochemistry (IHC): IHC : 1:500-1:2000 Background Information eEF1A2 is one of two isoforms (eEF1A1 and eEF1A2) of eukaryotic elongation factor 1 alpha (eEF1A or eEF1α). The two isoforms share more than 90% sequence identity and have the similar function that being a protein translation factor involved in protein synthesis. Addition, eEF1A2 plays an important role in cell cycle regulation, heat-shock response, aging, posttranslational modifications, and signal transduction (PMID: 25905039). eEF1A2 is expressed in heart, brain tissue, diaphragm and skeletal muscle while eEF1A1 is expressed ubiquitously (PMID:14588074). And the eEF1A2 protein stimulates the phospholipid signaling and activates the Akt-dependent cell migration and actin remodeling that ultimately favors tumorigenesis. It is reported that eEF1A2 was related with kinds of cancers such as ovarian cancer, prostate cancer, pancreatic cancer, breast cancer and lung cancer (PMID:14588074; 24853801; 25744894; 25905039). An 48 kDa band has also been reported(PMID: 24285179). Specification Tested Reactivity: human, mouse, rat, pig Host / Isotype: Mouse / IgG1 Class: Monoclonal Type: Antibody Immunogen: CatNo: Ag6875 Product name: Recombinant human EEF1A2 protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 109-463 aa of BC000432 Sequence: ADCAVLIVAAGVGEFEAGISKNGQTREHALLAYTLGVKQLIVGVNKMDSTEPAYSEKRYDEIVKEVSAYIKKIGYNPATVPFVPISGWHGDNMLEPSPNMPWFKGWKVERKEGNASGVSLLEALDTILPPTRPTDKPLRLPLQDVYKIGGIGTVPVGRVETGILRPGMVVTFAPVNITTEVKSVEMHHEALSEALPGDNVGFNVKNVSVKDIRRGNVCGDSKSDPPQEAAQFTSQVIILNHPGQISAGYSPVIDCHTAHIACKFAELKEKIDRRSGKKLEDNPKSLKSGDAAIVEMVPGKPMCVESFSQYPPLGRFAVRDMRQTVAVGVIKNVEKKSGGAGKVTKSAQKAQKAGK Predict reactive species Full Name: eukaryotic translation elongation factor 1 alpha 2 Calculated Molecular Weight: 50 kDa Observed Molecular Weight: 48-50 kDa GenBank Accession Number: BC000432 Gene Symbol: EEF1A2 Gene ID (NCBI): 1917 RRID: AB_2882149 Conjugate: Unconjugated Form: Liquid Purification Method: Protein G purification UNIPROT ID: Q05639 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.