Proteintech, 66883-1-Ig, CD98/SLC3A2 Monoclonal antibody

Size: 20ul / 150ul The CD98/SLC3A2 (66883-1-Ig) by Proteintech is a Monoclonal antibody targeting CD98/SLC3A2 in WB, IHC, IF/ICC, ELISA applications with reactivity to human samples 66883-1-Ig targets CD98/SLC3A2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: HeLa cells, MCF-7 cells, HepG2 cells, HT-29 cells, COLO 320 cells, Caco-2 cells, Jurkat cells Positive IHC detected in: human renal cell carcinoma tissue, human colon cancer tissue, human cervical cancer tissue, human liver cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HeLa cells Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Immunohistochemistry (IHC): IHC : 1:500-1:2000 Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600 Background Information CD98 is a cell-surface heterodimer consisting of a heavy chain (CD98hc) and a light chain. CD98hc also interacts with certain integrin b-subunits to regulate cell migration, survival, proliferation, and adhesion/polarity. CD98hc is overexpressed on the cell surface of many cancers and increased CD98hc expression is associated with the development and progression of tumors.(PMID:25084765). This protein has 4 isoforms with the molecular mass of 58-71 kDa and can be detected 85-94 kDa due to glycosylation. The glycosylated CD98hc can links to a non-glycosylated light chain (~40 kDa) via a disulfide bond to form a heterodimeric CD98 antigen with molecular mass of 120-130 kDa (PMID: 14770309). Specification Tested Reactivity: human Cited Reactivity: human Host / Isotype: Mouse / IgG2a Class: Monoclonal Type: Antibody Immunogen: CatNo: Ag7301 Product name: Recombinant human SLC3A2 protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 269-630 aa of BC001061 Sequence: KDDVAQTDLLQIDPNFGSKEDFDSLLQSAKKKSIRVILDLTPNYRGENSWFSTQVDTVATKVKDALEFWLQAGVDGFQVRDIENLKDASSFLAEWQNITKGFSEDRLLIAGTNSSDLQQILSLLESNKDLLLTSSYLSDSGSTGEHTKSLVTQYLNATGNRWCSWSLSQARLLTSFLPAQLLRLYQLMLFTLPGTPVFSYGDEIGLDAAALPGQPMEAPVMLWDESSFPDIPGAVSANMTVKGQSEDPGSLLSLFRRLSDQRSKERSLLHGDFHAFSAGPGLFSYIRHWDQNERFLVVLNFGDVGLSAGLQASDLPASASLPAKADLLLSTQPGREEGSPLELERLKLEPHEGLLLRFPYAA Predict reactive species Full Name: solute carrier family 3 (activators of dibasic and neutral amino acid transport), member 2 Calculated Molecular Weight: 68 kDa Observed Molecular Weight: 85-94 kDa, 120-130 kDa GenBank Accession Number: BC001061 Gene Symbol: CD98 Gene ID (NCBI): 6520 RRID: AB_2882215 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: P08195 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.