Proteintech, 67001-1-Ig, NUP50 Monoclonal antibody

Size: 20ul / 150ul The NUP50 (67001-1-Ig) by Proteintech is a Monoclonal antibody targeting NUP50 in WB, IHC, ELISA applications with reactivity to human, mouse samples 67001-1-Ig targets NUP50 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples. Tested Applications Positive WB detected in: HCT 116 cells, HeLa cells, HEK-293 cells, Ramos cells, Daudi cels, Sp2/0 cells, MCF-7 cells, HT-29 cells, U2OS cells, HepG2 cells, Jurkat cells, MOLT-4 cells, K-562 cells Positive IHC detected in: human breast cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Immunohistochemistry (IHC): IHC : 1:500-1:2000 Background Information Nup50 (also known as Npap60) is a nucleoporin that binds directly to importin α and their interaction plays an important role in nuclear import. Nup50 is a mobile nucleoporin localized primarily in the nuclear basket of the nuclear pore complex (NPC) and in the nucleoplasm. Human Nup50 has two isoforms: Npap60L (1-469 aa), and its alternatively spliced isoform, Npap60S (29-469 aa) of Npap60L. Specification Tested Reactivity: human, mouse Host / Isotype: Mouse / IgG1 Class: Monoclonal Type: Antibody Immunogen: CatNo: Ag15144 Product name: Recombinant human NUP50 protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 141-440 aa of BC028125 Sequence: DWIVKHVNTNPLCDLTPIFKDYEKYLANIEQQHGNSGRNSESESNKVAAETQSPSLFGSTKLQQESTFLFHGNKTEDTPDKKMEVASEKKTDPSSLGATSASFNFGKKVDSSVLGSLSSVPLTGFSFSPGNSSLFGKDTTQSKPVSSPFPTKPLEGQAEGDSGECKGGDEEENDEPPKVVVTEVKEEDAFYSKKCKLFYKKDNEFKEKGIGTLHLKPTANQKTQLLVRADTNLGNILLNVLIPPNMPCTRTGKNNVLIVCVPNPPIDEKNATMPVTMLIRVKTSEDADELHKILLEKKDA Predict reactive species Full Name: nucleoporin 50kDa Calculated Molecular Weight: 468 aa, 50 kDa Observed Molecular Weight: 50-55 kDa GenBank Accession Number: BC028125 Gene Symbol: NUP50 Gene ID (NCBI): 10762 RRID: AB_2882318 Conjugate: Unconjugated Form: Liquid Purification Method: Protein G purification UNIPROT ID: Q9UKX7 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.