Proteintech, 67057-1-Ig, ATX3,ATXN3 Monoclonal antibody

Size: 20ul / 150ul The ATX3,ATXN3 (67057-1-Ig) by Proteintech is a Monoclonal antibody targeting ATX3,ATXN3 in WB, IHC, IF/ICC, ELISA applications with reactivity to Human, mouse, pig samples 67057-1-Ig targets ATX3,ATXN3 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, mouse, pig samples. Tested Applications Positive WB detected in: MCF-7 cells, HeLa cells, Jurkat cells, HEK-293 cells, pig brain tissue, mouse brain tissue Positive IHC detected in: human pancreas cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: A549 cells Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Immunohistochemistry (IHC): IHC : 1:500-1:2000 Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600 Background Information ATXN3, which has deubiquitinase activity and act as a component of the ubiquitin proteasome system, plays a role in transcriptional regulation and neuroprotection. ATXN3 interacts with RAD23, HHR23A and HHR23B, involves in the pathology of MJD. ATXN3 is a mixed-linkage, chain-editing enzyme and that the UIM region of ATXN3 regulates its substrate specificity. Contains an N-terminal deubiquitinating domain, called the Josephin domain, followed by 2 ubiquitin-interacting motifs (UIMs) and a polyQ tract near the C terminus. ATXN3 can be phosphorylated in a protein casein kinase-2-dependent manner, thus the MW would be larger than the predicted one. Specification Tested Reactivity: Human, mouse, pig Cited Reactivity: human, mouse Host / Isotype: Mouse / IgG2a Class: Monoclonal Type: Antibody Immunogen: CatNo: Ag6627 Product name: Recombinant human ATX3,ATXN3 protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 1-370 aa of BC033711 Sequence: MESIFHEKQEGSLCAQHCLNNLLQGEYFSPVELSSIAHQLDEEERMRMAEGGVTSEDYRTFLQQPSGNMDDSGFFSIQVISNALKVWGLELILFNSPEYQRLRIDPINERSFICNYKEHWFTVRKLGKQWFNLNSLLTGPELISDTYLALFLAQLQQEGYSIFVVKGDLPDCEADQLLQMIRVQQMHRPKLIGEELAQLKEQRVHKTDLERVLEANDGSGMLDEDEEDLQRALALSRQEIDMEDEEADLRRAIQLSMQGSSRNISQDMTQTSGTNLTSEELRKRREAYFEKQQQKQQQQQQQQQQQQQQQQQQQGDLSGQSSHPCERPATSSGALGSDLGDAMSEEDMLQAAVTMSLETVRNDLKTEGKK Predict reactive species Full Name: ataxin 3 Calculated Molecular Weight: 370 aa, 43 kDa Observed Molecular Weight: 41-45 kDa GenBank Accession Number: BC033711 Gene Symbol: ATXN3 Gene ID (NCBI): 4287 RRID: AB_2882368 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: P54252 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.