Proteintech, 67077-1-Ig, APBB1 Monoclonal antibody

Size: 20ul / 150ul The APBB1 (67077-1-Ig) by Proteintech is a Monoclonal antibody targeting APBB1 in WB, IHC, IF-P, ELISA applications with reactivity to Human, Mouse, Rat, Pig samples 67077-1-Ig targets APBB1 in WB, IHC, IF-P, ELISA applications and shows reactivity with Human, Mouse, Rat, Pig samples. Tested Applications Positive WB detected in: pig brain tissue, Pig brain, Rat brain, Mouse brain Positive IHC detected in: mouse cerebellum tissue, mouse brain tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF-P detected in: mouse brain tissue Recommended dilution Western Blot (WB): WB : 1:1000-1:3000 Immunohistochemistry (IHC): IHC : 1:50-1:500 Immunofluorescence (IF)-P: IF-P : 1:200-1:800 Background Information APBB1(Amyloid-beta A4 precursor protein-binding family B member 1) encoded FE65 protein. It was known as a binding partner of APP in the Alzheimer's disease studies, and expressed at high levels in brain especially in cerebellum, hippocampus, and cortex. FE65 and FE65-like (FE65L or FE65L1) proteins are cytoplasmic adaptor proteins that possess two phosphotyrosine binding domains (PTB1 and PTB2) and one WW binding domain (PMID:22429478). After phosphorylation modification, the band of FE65 protein would appear around 100 kDa. However, some tested a non-specific band at 55-60 kDa, it was refer to as FE65-like protein(PMID:12843239). Specification Tested Reactivity: Human, Mouse, Rat, Pig Host / Isotype: Mouse / IgG1 Class: Monoclonal Type: Antibody Immunogen: CatNo: Ag27436 Product name: Recombinant human APBB1 protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 43-268 aa of BC010854 Sequence: TAVGPKDLRSAMGEGGGPEPGPANAKWLKEGQNQLRRAATAHRDQNRNVTLTLAEEASQEPEMAPLGPKGLIHLYSELELSAHNAANRGLRGPGLIISTQEQGPDEGEEKAAGEAEEEEEDDDDEEEEEDLSSPPGLPEPLESVEAPPRPQALTDGPREHSKSASLLFGMRNSAASDEDSSWATLSQGSPSYGSPEDTDSFWNPNAFETDSDLPAGWMRVQDTSGT Predict reactive species Full Name: amyloid beta (A4) precursor protein-binding, family B, member 1 (Fe65) Calculated Molecular Weight: 708 aa, 77 kDa Observed Molecular Weight: 100 kDa GenBank Accession Number: BC010854 Gene Symbol: APBB1 Gene ID (NCBI): 322 RRID: AB_2882385 Conjugate: Unconjugated Form: Liquid Purification Method: Protein G purification UNIPROT ID: O00213 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.