Proteintech, 67103-1-Ig, MCM4 Monoclonal antibody

Size: 20ul / 150ul The MCM4 (67103-1-Ig) by Proteintech is a Monoclonal antibody targeting MCM4 in WB, IF/ICC, ELISA applications with reactivity to human, mouse, rat samples 67103-1-Ig targets MCM4 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: HeLa cells, HSC-T6 cells, NIH/3T3 cells, HEK-293 cells, A431 cells, HL-60 cells, Jurkat cells Positive IF/ICC detected in: U2OS cells Recommended dilution Western Blot (WB): WB : 1:5000-1:20000 Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600 Background Information DNA replication licensing factor MCM4 (MCM4), also named Cdc21, acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human, mouse Host / Isotype: Mouse / IgG2b Class: Monoclonal Type: Antibody Immunogen: CatNo: Ag28642 Product name: Recombinant human MCM4 protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 515-859 aa of BC031061 Sequence: SKSQLLQYVYNLVPRGQYTSGKGSSAVGLTAYVMKDPETRQLVLQTGALVLSDNGICCIDEFDKMNESTRSVLHEVMEQQTLSIAKAGIICQLNARTSVLAAANPIESQWNPKKTTIENIQLPHTLLSRFDLIFLMLDPQDEAYDRRLAHHLVALYYQSEEQAEEELLDMAVLKDYIAYAHSTIMPRLSEEASQALIEAYVDMRKIGSSRGMVSAYPRQLESLIRLAEAHAKVRLSNKVEAIDVEEAKRLHREALKQSATDPRTGIVDISILTTGMSATSRKRKEELAEALKKLILSKGKTPALKYQQLFEDIRGQSDIAITKDMFEEALRALADDDFLTVTGKT Predict reactive species Full Name: minichromosome maintenance complex component 4 Calculated Molecular Weight: 863 aa, 97 kDa Observed Molecular Weight: 97 kDa GenBank Accession Number: BC031061 Gene Symbol: MCM4 Gene ID (NCBI): 4173 RRID: AB_2882408 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: P33991 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.