Proteintech, 67177-1-Ig, SHARPIN Monoclonal antibody

Size: 20ul / 150ul The SHARPIN (67177-1-Ig) by Proteintech is a Monoclonal antibody targeting SHARPIN in WB, ELISA applications with reactivity to Human samples 67177-1-Ig targets SHARPIN in WB, ELISA applications and shows reactivity with Human samples. Tested Applications Positive WB detected in: A431 cells, HeLa cells, A549 cells, HepG2 cells, HEK-293 cells, Jurkat cells, K-562 cells Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Background Information SHARPIN is as a third component of the linear ubiquitin chain assembly complex (LUBAC), recruited to the CD40 and TNF receptor signaling complexes together with its other constituents, HOIL1 and HOIP(PMID:21455173).SHARPIN is enriched in the postsynaptic density and forms a complex with SHANK in heterologous cells and brain(PMID:11178875).It has 2 isoforms produced by alternative splicing. Specification Tested Reactivity: Human Host / Isotype: Mouse / IgG2a Class: Monoclonal Type: Antibody Immunogen: CatNo: Ag6367 Product name: Recombinant human SHARPIN protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 1-326 aa of BC025244 Sequence: MAPPAGGAAAAASDLGSAAVLLAVHAAVRPLGAGPDAEAQLRRLQLSADPERPGRFRLELLGAGPGAVNLEWPLESVSYTIRGPTQHELQPPPGGPGTLSLHFLNPQEAQRWAVLVRGATVEGQNGSKSNSPPALGPEACPVSLPSPPEASTLKGPPPEADLPRSPGNLTEREELAGSLARAIAGGDEKGAAQVAAVLAQHRVALSVQLQEACFPPGPIRLQVTLEDAASAASAASSAHVALQVHPHCTVAALQEQVFSELGFPPAVQRWVIGRCLCVPERSLASYGVRQDGDPAFLYLLSAPREAPAQLVLSFLHLHQCPRPPWL Predict reactive species Full Name: SHANK-associated RH domain interactor Calculated Molecular Weight: 40 kDa Observed Molecular Weight: 40 kDa GenBank Accession Number: BC025244 Gene Symbol: SHARPIN Gene ID (NCBI): 81858 RRID: AB_2882473 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: Q9H0F6 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.