Proteintech, 67341-1-Ig, ATG7 Monoclonal antibody

Size: 20ul / 150ul The ATG7 (67341-1-Ig) by Proteintech is a Monoclonal antibody targeting ATG7 in WB, IHC, IF/ICC, FC (Intra), ELISA applications with reactivity to human, mouse, rat, pig samples 67341-1-Ig targets ATG7 in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat, pig samples. Tested Applications Positive WB detected in: LNCaP cells, Jurkat cells, pig spleen tissue, HepG2 cells, 4T1 cells, HeLa cells, THP-1 cells, HSC-T6 cells, NIH/3T3 cells, HEK-293 cells Positive IHC detected in: human cervical cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HeLa cells Positive FC (Intra) detected in: HeLa cells Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Immunohistochemistry (IHC): IHC : 1:500-1:2000 Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500 Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.50 ug per 10^6 cells in a 100 µl suspension Background Information Atg7 is an E1-like enzyme that is specifically involved in autophagosome formation and is essential for autophagy. As an autophagic-related protein it is required for linking to Atg12, Atg5 and Atg8, which are essential for Atg conjugation and autophagosome formation. Atg7 has been reported as an important regulator of autophagy with starvation-induced or chemotherapeutic agent treatment. The high expression level of ATG7 is related to the survival of patients with breast cancer. There are several isoforms of ATG7 protein ranged from 68 kDa to 78 kDa. Specification Tested Reactivity: human, mouse, rat, pig Cited Reactivity: human, mouse, rat, canine Host / Isotype: Mouse / IgG2a Class: Monoclonal Type: Antibody Immunogen: CatNo: Ag29172 Product name: Recombinant human ATG7 protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 451-676 aa of BC000091 Sequence: LEQARRDVEQLEQLIESHDVVFLLMDTRESRWLPAVIAASKRKLVINAALGFDTFVVMRHGLKKPKQQGAGDLCPNHPVASADLLGSSLFANIPGYKLGCYFCNDVVAPGDSTRDRTLDQQCTVSRPGLAVIAGALAVELMVSVLQHPEGGYAIASSSDDRMNEPPTSLGLVPHQVLDQYEREGFNFLAKVFNSSHSFLEDLTGLTLLHQETQAAEIWDMSDDETI Predict reactive species Full Name: ATG7 autophagy related 7 homolog (S. cerevisiae) Calculated Molecular Weight: 78 kDa Observed Molecular Weight: 68-78 kDa GenBank Accession Number: BC000091 Gene Symbol: ATG7 Gene ID (NCBI): 10533 RRID: AB_2882599 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: O95352 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.