Proteintech, 67354-1-Ig, HMG20B Monoclonal antibody

Size: 20ul / 150ul The HMG20B (67354-1-Ig) by Proteintech is a Monoclonal antibody targeting HMG20B in WB, IHC, ELISA applications with reactivity to human, mouse, rat samples 67354-1-Ig targets HMG20B in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: LNCaP cells, HeLa cells, HEK-293 cells, Jurkat cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells Positive IHC detected in: human skin cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Immunohistochemistry (IHC): IHC : 1:1000-1:4000 Background Information CoREST (corepressor of repressor element-1-silencing transcription factor) is a chromatin- modifying complex that was first identified as a regulator of neuronal gene expression, having a essential role in neuronal differentiation (PMID:10449787). The CoREST complex is recruited by REST (RE1-silencing transcription factor) that recognizes the 21-nucleotide RE-1 sequence in the promoter of more than 1,700 different target genes in human and mouse (PMID:15240883). HMGB20B is one component of the CoREST complex, which mediates chromatin remodeling and repression of specific genes involved in neuronal differentiation through modulation of chromatin structure (PMID:19997604). The predicted molecular weight of the HMG20B protein is 36 kDa, while the molecular weight detected in the literature is approximately 40 kDa by SDS-PAGE (PMID: 36171271). Specification Tested Reactivity: human, mouse, rat Host / Isotype: Mouse / IgG1 Class: Monoclonal Type: Antibody Immunogen: CatNo: Ag6103 Product name: Recombinant human HMG20B protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 73-284 aa of BC002552 Sequence: PVTGYVRFLNERREQIRTRHPDLPFPEITKMLGAEWSKLQPTEKQRYLDEAEREKQQYMKELRAYQQSEAYKMCTEKIQEKKIKKEDSSSGLMNTLLNGHKGGDCDGFSTFDVPIFTEEFLDQNKAREAELRRLRKMNVAFEEQNAVLQRHTQSMSSARERLEQELALEERRTLALQQQLQAVRQALTASFASLPVPGTGETPTLGTLDFYM Predict reactive species Full Name: high-mobility group 20B Calculated Molecular Weight: 36 kDa Observed Molecular Weight: 40 kDa GenBank Accession Number: BC002552 Gene Symbol: HMG20B Gene ID (NCBI): 10362 RRID: AB_2882609 Conjugate: Unconjugated Form: Liquid Purification Method: Protein G purification UNIPROT ID: Q9P0W2 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.