Proteintech, 67355-1-Ig, P27; KIP1 Monoclonal antibody

Size: 20ul / 150ul The P27; KIP1 (67355-1-Ig) by Proteintech is a Monoclonal antibody targeting P27; KIP1 in WB, ELISA applications with reactivity to Human, mouse, rat samples 67355-1-Ig targets P27; KIP1 in WB, ELISA applications and shows reactivity with Human, mouse, rat samples. Tested Applications Positive WB detected in: Jurkat cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells Recommended dilution Western Blot (WB): WB : 1:5000-1:10000 Background Information DKN1B, also named as P27 or KIP1, is a cyclin-dependent kinase inhibitor, which shares a limited similarity with CDK inhibitor CDKN1A/p21. P27 binds to and prevents the activation of cyclin E-CDK2 or cyclin D-CDK4 complexes, and thus controlling cell cycle progression at G1. The degradation of this protein, which is triggered by its CDK dependent phosphorylation and subsequent ubiquitination by SCF complexes, is required for the cellular transition from quiescence to the proliferative state. Downregulation of P27 has been implicated in the progression of several malignancies, including lung cancer, hepatocellular carcinoma, salivary cancer, oral squamous cell carcinomas, and gastric cancer. Specification Tested Reactivity: Human, mouse, rat Cited Reactivity: human, mouse Host / Isotype: Mouse / IgG1 Class: Monoclonal Type: Antibody Immunogen: CatNo: Ag14634 Product name: Recombinant human P27; KIP1 protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 1-198 aa of BC001971 Sequence: MSNVRVSNGSPSLERMDARQAEHPKPSACRNLFGPVDHEELTRDLEKHCRDMEEASQRKWNFDFQNHKPLEGKYEWQEVEKGSLPEFYYRPPRPPKGACKVPAQESQDGSGSRPAAPLIGAPANSEDTHLVDPKTDPSDSQTGLAEQCAGIRKRPATDDSSTQNKRANRTEENVSDGSPNAGSVEQTPKKPGLRRRQT Predict reactive species Full Name: cyclin-dependent kinase inhibitor 1B (p27, Kip1) Calculated Molecular Weight: 198 aa, 22 kDa Observed Molecular Weight: 27 kDa GenBank Accession Number: BC001971 Gene Symbol: p27 Kip1/CDKN1B Gene ID (NCBI): 1027 RRID: AB_2882610 Conjugate: Unconjugated Form: Liquid Purification Method: Protein G purification UNIPROT ID: P46527 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.