Proteintech, 67388-1-Ig, PTPN2 Monoclonal antibody

Size: 20ul / 150ul The PTPN2 (67388-1-Ig) by Proteintech is a Monoclonal antibody targeting PTPN2 in WB, IHC, ELISA applications with reactivity to human, mouse, rat samples 67388-1-Ig targets PTPN2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: PC-3 cells, HeLa cells, 4T1 cells, LNCaP cells, HEK-293T cells, MCF-7 cells, Jurkat cells, HSC-T6 cells, NIH/3T3 cells, HepG2 cells, K-562 cells Positive IHC detected in: human breast cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Immunohistochemistry (IHC): IHC : 1:250-1:1000 Background Information PTPN2(Tyrosine-protein phosphatase non-receptor type 2) is also named as PTPT and belongs to the protein-tyrosine phosphatase family. It may modulate beta-cell apoptosis and/or local release of inflammatory mediators in the course of a viral infection by acting, at least in part, at the pancreatic beta-cell level(PMID: 19825843). This protein has some isoforms produced by alternative splicing with the molecular mass of 40-50 kDa. Specification Tested Reactivity: human, mouse, rat Host / Isotype: Mouse / IgG1 Class: Monoclonal Type: Antibody Immunogen: CatNo: Ag29749 Product name: Recombinant human PTPN2 protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 1-346 aa of BC016727 Sequence: MPTTIEREFEELDTQRRWQPLYLEIRNESHDYPHRVAKFPENRNRNRYRDVSPYDHSRVKLQNAENDYINASLVDIEEAQRSYILTQGPLPNTCCHFWLMVWQQKTKAVVMLNRIVEKESVKCAQYWPTDDQEMLFKETGFSVKLLSEDVKSYYTVHLLQLENINSGETRTISHFHYTTWPDFGVPESPASFLNFLFKVRESGSLNPDHGPAVIHCSAGIGRSGTFSLVDTCLVLMEKGDDINIKQVLLNMRKYRMGLIQTPDQLRFSYMAIIEGAKCIKGDSSIQKRWKELSKEDLSPAFDHSPNKIMTEKYNGNRIGLEEEKLTGDRCTGLSSKMQDTMEENSE Predict reactive species Full Name: protein tyrosine phosphatase, non-receptor type 2 Calculated Molecular Weight: 49 kDa Observed Molecular Weight: 45-50 kDa GenBank Accession Number: BC016727 Gene Symbol: PTPN2 Gene ID (NCBI): 5771 RRID: AB_2882632 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: P17706 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.