Proteintech, 67433-1-Ig, CYP20A1 Monoclonal antibody

Size: 20ul / 150ul The CYP20A1 (67433-1-Ig) by Proteintech is a Monoclonal antibody targeting CYP20A1 in WB, IHC, IF/ICC, ELISA applications with reactivity to human, mouse, rat samples 67433-1-Ig targets CYP20A1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: LNCaP cells, HeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells, HSC-T6 cells, NIH/3T3 cells, 4T1 cells, K-562 cells, Neuro-2a cells Positive IHC detected in: human liver cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HeLa cells Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Immunohistochemistry (IHC): IHC : 1:250-1:1000 Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800 Specification Tested Reactivity: human, mouse, rat Host / Isotype: Mouse / IgG1 Class: Monoclonal Type: Antibody Immunogen: CatNo: Ag9715 Product name: Recombinant human CYP20A1 protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 112-462 aa of BC020616 Sequence: SGGGSVSENHMREKLYENGVTDSLKSNFALLLKLSEELLDKWLSYPETQHVPLSQHMLGFAMKSVTQMVMGSTFEDDQEVIRFQKNHGTVWSEIGKGFLDGSLDKNMTRKKQYEDALMQLESVLRNIIKERKGRNFSQHIFIDSLVQGNLNDQQILEDSMIFSLASCIITAKLCTWAICFLTTSEEVQKKLYEEINQVFGNGPVTPEKIEQLRYCQHVLCETVRTAKLTPVSAQFQDIEGKIDRFIIPRETLVLYALGVVLQDPNTWPSPHKFDPDRFDDELVMKTFSSLGFSGTQECPELRFAYMVTTVLLSVLVKRLHLLSVEGQVIETKYELVTSSREEAWITVSKRY Predict reactive species Full Name: cytochrome P450, family 20, subfamily A, polypeptide 1 Calculated Molecular Weight: 462 aa, 52 kDa Observed Molecular Weight: 49-52 kDa GenBank Accession Number: BC020616 Gene Symbol: CYP20A1 Gene ID (NCBI): 57404 RRID: AB_2882671 Conjugate: Unconjugated Form: Liquid Purification Method: Protein G purification UNIPROT ID: Q6UW02 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.