Proteintech, 67481-1-Ig, FURIN Monoclonal antibody

Size: 20ul / 150ul The FURIN (67481-1-Ig) by Proteintech is a Monoclonal antibody targeting FURIN in WB, IHC, ELISA applications with reactivity to Human, Mouse samples 67481-1-Ig targets FURIN in WB, IHC, ELISA applications and shows reactivity with Human, Mouse samples. Tested Applications Positive WB detected in: A549 cells, COLO 320 cells, NIH/3T3 cells, JAR cells, HeLa cells Positive IHC detected in: human placenta tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:1000-1:5000 Immunohistochemistry (IHC): IHC : 1:500-1:2000 Background Information FURIN, also named as FUR, PACE, PCSK3, SPC1, Kex2p, is likely to represent the ubiquitous endoprotease activity within constitutive secretory pathways and capable of cleavage at the RX(K/R)R consensus motif. Furin is synthesized as an inactive zymogen that may minimize the occurrence of premature enzymatic activity that would lead to alternative protein activation or degradation. Glycosylation and other post-translational modifications can make the pre-pro furin run at 100-110 kDa, the mature furin at 98-95 kDa and shed furin at 90 kDa. Specification Tested Reactivity: Human, Mouse Host / Isotype: Mouse / IgG1 Class: Monoclonal Type: Antibody Immunogen: CatNo: Ag13313 Product name: Recombinant human FURIN protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 495-794 aa of BC012181 Sequence: YNRRGDLAIHLVSPMGTRSTLLAARPHDYSADGFNDWAFMTTHSWDEDPSGEWVLEIENTSEANNYGTLTKFTLVLYGTAPEGLPVPPESSGCKTLTSSQACVVCEEGFSLHQKSCVQHCPPGFAPQVLDTHYSTENDVETIRASVCAPCHASCATCQGPALTDCLSCPSHASLDPVEQTCSRQSQSSRESPPQQQPPRLPPEVEAGQRLRAGLLPSHLPEVVAGLSCAFIVLVFVTVFLVLQLRSGFSFRGVKVYTMDRGLISYKGLPPEAWQEECPSDSEEDEGRGERTAFIKDQSAL Predict reactive species Full Name: furin (paired basic amino acid cleaving enzyme) Calculated Molecular Weight: 87 kDa Observed Molecular Weight: 90-110 kDa GenBank Accession Number: BC012181 Gene Symbol: FURIN Gene ID (NCBI): 5045 RRID: AB_2882708 Conjugate: Unconjugated Form: Liquid Purification Method: Protein G purification UNIPROT ID: P09958 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.