Product Description
Size: 20ul / 150ul
The AKR1B1 (67498-1-Ig) by Proteintech is a Monoclonal antibody targeting AKR1B1 in WB, IHC, IF/ICC, ELISA applications with reactivity to human, mouse samples
67498-1-Ig targets AKR1B1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.
Tested Applications
Positive WB detected in: HEK-293 cells, HeLa cells, HepG2 cells, Jurkat cells, NIH/3T3 cells, 4T1 cells
Positive IHC detected in: human bladder tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0
Positive IF/ICC detected in: BxPC-3 cells
Recommended dilution
Western Blot (WB): WB : 1:2000-1:10000
Immunohistochemistry (IHC): IHC : 1:250-1:1000
Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800
Specification
Tested Reactivity: human, mouse
Cited Reactivity: human, mouse
Host / Isotype: Mouse / IgG1
Class: Monoclonal
Type: Antibody
Immunogen: CatNo: Ag7571 Product name: Recombinant human AKR1B1 protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 1-316 aa of BC000260 Sequence: MASRLLLNNGAKMPILGLGTWKSPPGQVTEAVKVAIDVGYRHIDCAHVYQNENEVGVAIQEKLREQVVKREELFIVSKLWCTYHEKGLVKGACQKTLSDLKLDYLDLYLIHWPTGFKPGKEFFPLDESGNVVPSDTNILDTWAAMEELVDEGLVKAIGISNFNHLQVEMILNKPGLKYKPAVNQIECHPYLTQEKLIQYCQSKGIVVTAYSPLGSPDRPWAKPEDPSLLEDPRIKAIAAKHNKTTAQVLIRFPMQRNLVVIPKSVTPERIAENFKVFDFELSSQDMTTLLSYNRNWRVCALLSCTSHKDYPFHEEF Predict reactive species
Full Name: aldo-keto reductase family 1, member B1 (aldose reductase)
Calculated Molecular Weight: 36 kDa
Observed Molecular Weight: 34 kDa
GenBank Accession Number: BC000260
Gene Symbol: AKR1B1
Gene ID (NCBI): 231
RRID: AB_2882722
Conjugate: Unconjugated
Form: Liquid
Purification Method: Protein G purification
UNIPROT ID: P15121
Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.
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Collaboration
Tony Tang
Email: Tony.Tang@iright.com
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