Proteintech, 67558-1-Ig, Phospho-GSK3B (Ser9) Monoclonal antibody

Size: 20ul / 100ul The Phospho-GSK3B (Ser9) (67558-1-Ig) by Proteintech is a Monoclonal antibody targeting Phospho-GSK3B (Ser9) in WB, IHC, IF/ICC, ELISA applications with reactivity to human samples 67558-1-Ig targets Phospho-GSK3B (Ser9) in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: HeLa cells, Calyculin A treated PC-3 cells, Calyculin A treated HEK-293T cells Positive IHC detected in: human liver cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: Calyculin A treated PC-3 cells Recommended dilution Western Blot (WB): WB : 1:2000-1:10000 Immunohistochemistry (IHC): IHC : 1:150-1:600 Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500 Background Information Glycogen synthase kinase-3 (GSK3) is a proline-directed serine-threonine kinase that was initially identified as a phosphorylating and inactivating glycogen synthase .GSK3B is involved in energy metabolism, neuronal cell development, and body pattern formation.In skeletal muscle, it contributes to INS regulation of glycogen synthesis by phosphorylating and inhibiting GYS1 activity and hence glycogen synthesis. Researches showed that the crystal structure of human GSK3B, expressed in insect cells, at 2.8-angstrom resolution . Specification Tested Reactivity: human Cited Reactivity: human, mouse, rat, chicken, bovine Host / Isotype: Mouse / IgG1 Class: Monoclonal Type: Antibody Immunogen: Peptide Predict reactive species Full Name: glycogen synthase kinase 3 beta Observed Molecular Weight: 48 kDa GenBank Accession Number: NM_002093 Gene Symbol: GSK3B Gene ID (NCBI): 2932 RRID: AB_2882772 Conjugate: Unconjugated Form: Liquid Purification Method: Protein G purification UNIPROT ID: P49841 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.