Proteintech, 67579-1-Ig, ETV6 Monoclonal antibody

Size: 20ul / 150ul The ETV6 (67579-1-Ig) by Proteintech is a Monoclonal antibody targeting ETV6 in WB, IHC, ELISA applications with reactivity to Human samples 67579-1-Ig targets ETV6 in WB, IHC, ELISA applications and shows reactivity with Human samples. Tested Applications Positive WB detected in: Jurkat cells, U2OS cells, A431 cells, K-562 cells, THP-1 cells Positive IHC detected in: human breast cancer tissue, human lung cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:1000-1:6000 Immunohistochemistry (IHC): IHC : 1:50-1:500 Background Information ETV6(ETS Variant Transcription Factor 6), also known as TEL, is a Protein Coding gene. ETV6 is a member of the ETS family of transcription factors encoded by the ETS variant 6 gene (ETV6), located on chromosome 12p13.2. ETV6 protein contains 3 domains: a) the pointed N-terminal (i.e. PNT) domain which forms oligomer partners with itself as well as other transcription factors (e.g. FLI1) and is required for ETV6's transcriptional repressing activity; b) the central regulatory domain; and c) the C-terminal DNA-binding domain(PMID: 22578774). ETV6 is highly expressed in early hematopoietic progenitor cells(HPCs) and is essential for hematopoiesis in the bone marrow(PMID: 31248877). Specification Tested Reactivity: Human Host / Isotype: Mouse / IgG1 Class: Monoclonal Type: Antibody Immunogen: CatNo: Ag30054 Product name: Recombinant human ETV6 protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 150-312 aa of BC043399 Sequence: QNHEEDNCVQRTPRPSVDNVHHNPPTIELLHRSRSPITTNHRPSPDPEQRPLRSPLDNMIRRLSPAERAQGPRPHQENNHQESYPLSVSPMENNHCPASSESHPKPSSPRQESTRVIQLMPSPIMHPLILNPRHSVDFKQSRLSEDGLHREGKPINLSHREDL Predict reactive species Full Name: ets variant 6 Calculated Molecular Weight: 452 aa, 53 kDa Observed Molecular Weight: 60 kDa GenBank Accession Number: BC043399 Gene Symbol: ETV6 Gene ID (NCBI): 2120 RRID: AB_2918493 Conjugate: Unconjugated Form: Liquid Purification Method: Protein G purification UNIPROT ID: P41212 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.