Proteintech, 67632-1-Ig, PPID Monoclonal antibody

Size: 20ul / 150ul The PPID (67632-1-Ig) by Proteintech is a Monoclonal antibody targeting PPID in WB, IHC, ELISA applications with reactivity to human, mouse, rat, pig samples 67632-1-Ig targets PPID in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat, pig samples. Tested Applications Positive WB detected in: Raji cells, Jurkat cells, K-562 cells, JAR cells, pig brain issue, rat brain tissue, mouse brain tissue Positive IHC detected in: human cervical cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Immunohistochemistry (IHC): IHC : 1:500-1:2000 Background Information PPID(Peptidyl-prolyl cis-trans isomerase D) is also named CYP40, CYPD, and belongs to the cyclophilin-type PPIase family, which accelerates the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. The inactivation of the Ppid gene rescued the disease phenotype of Col6a1 deficiency (PMID:19293339). This PPID antibody detected a strong band at an apparent molecular mass of 41 kDa and a weak band at 70 kDa due to crosslink (PMID: 33320090, 29097228). Specification Tested Reactivity: human, mouse, rat, pig Host / Isotype: Mouse / IgG1 Class: Monoclonal Type: Antibody Immunogen: CatNo: Ag4371 Product name: Recombinant human PPID protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 57-370 aa of BC030707 Sequence: GIGHTTGKPLHFKGCPFHRIIKKFMIQGGDFSNQNGTGGESIYGEKFEDENFHYKHDREGLLSMANAGRNTNGSQFFITTVPTPHLDGKHVVFGQVIKGIGVARILENVEVKGEKPAKLCVIAECGELKEGDDGGIFPKDGSGDSHPDFPEDADIDLKDVDKILLITEDLKNIGNTFFKSQNWEMAIKKYAEVLRYVDSSKAVIETADRAKLQPIALSCVLNIGACKLKMSNWQGAIDSCLEALELDPSNTKALYRRAQGWQGLKEYDQALADLKKAQGIAPEDKAIQAELLKVKQKIKAQKDKEKAVYAKMFA Predict reactive species Full Name: peptidylprolyl isomerase D Calculated Molecular Weight: 370 aa, 41 kDa Observed Molecular Weight: 38-41 kDa GenBank Accession Number: BC030707 Gene Symbol: PPID Gene ID (NCBI): 5481 RRID: AB_2882833 Conjugate: Unconjugated Form: Liquid Purification Method: Protein G purification UNIPROT ID: Q08752 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.