Proteintech, 67633-1-Ig, Maspin Monoclonal antibody

Size: 20ul / 150ul The Maspin (67633-1-Ig) by Proteintech is a Monoclonal antibody targeting Maspin in WB, IHC, IF/ICC, ELISA applications with reactivity to human samples 67633-1-Ig targets Maspin in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: HepG2 cells, HeLa cells, HUMAN saliva, A431 cells Positive IHC detected in: human oesophagus cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HEK-293 cells Recommended dilution Western Blot (WB): WB : 1:1000-1:6000 Immunohistochemistry (IHC): IHC : 1:150-1:600 Immunofluorescence (IF)/ICC: IF/ICC : 1:800-1:3200 Background Information SERPINB5, also named PI-5, Maspin, and Serpin B5, belongs to the serpin family and Ov-serpin subfamily. It is a tumor suppressor. It blocks the growth, invasion, and metastatic properties of mammary tumors. As it does not undergo the S (stressed) to R (relaxed) conformational transition characteristic of active serpins, SERPINB5 exhibits no serine protease inhibitory activity. Specification Tested Reactivity: human Host / Isotype: Mouse / IgG2a Class: Monoclonal Type: Antibody Immunogen: CatNo: Ag19129 Product name: Recombinant human SERPINB5 protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 1-231 aa of BC020713 Sequence: MDALQLANSAFAVDLFKQLCEKEPLGNVLFSPICLSTSLSLAQVGAKGDTANEIGQVLHFENVKDVPFGFQTVTSDVNKLSSFYSLKLIKRLYVDKSLNLSTEFISSTKRPYAKELETVDFKDKLEETKGQINNSIKDLTDGHFENILADNSVNDQTKILVVNAAYFVGKWMKKFPESETKECPFRVNKVCGAACSSKRSPIIDVKNDRDRVGHKSIPMRNLRARPAKCLS Predict reactive species Full Name: serpin peptidase inhibitor, clade B (ovalbumin), member 5 Calculated Molecular Weight: 375 aa, 42 kDa Observed Molecular Weight: 38-40 kDa GenBank Accession Number: BC020713 Gene Symbol: Maspin Gene ID (NCBI): 5268 RRID: AB_2882834 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: P36952 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.