Proteintech, 67637-1-Ig, CCDC6 Monoclonal antibody

Size: 20ul / 150ul The CCDC6 (67637-1-Ig) by Proteintech is a Monoclonal antibody targeting CCDC6 in WB, IF/ICC, ELISA applications with reactivity to human, mouse, rat samples 67637-1-Ig targets CCDC6 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: MCF-7 cells, A549 cells, HepG2 cells, U2OS cells, LNCaP cells, HeLa cells, K-562 cells, Jurkat cells, HSC-T6 cells, NIH/3T3 cells Positive IF/ICC detected in: MCF-7 cells Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800 Background Information CCDC6 (Coiled-coil domain-containing protein 6) is also named as Protein H4, D10S170 and TST1. CCDC6, was initially isolated as part of a tumorigenic DNA originated by the fusion of CCDC6 with the tyrosine kinase of RET receptor. CCDC6 has been considered as an accidental partner of the RET protooncogene, providing the promoter and the first 101 aa necessary for the constitutive activation of the oncogenic Tyrosine Kinase (TK) RET in thyroid cells. The 65 kDa product of CCDC6 has a nuclear transfer sequence with no transmembrane domains and is predicted to locate in both the nucleus and the cytoplasm (PMID: 29044514). The CCDC6 is a phosphoprotein, predicted target of several S/T kinases which can modulate the protein stability and the intracellular shuttling into the nucleus upon different cellular signals mediated by ERK1/2, ATM and CDK1/2. CCDC6 is involved in cellular response to DNA damage mediated by ATM, with the final result of promoting cellular apoptosis. CCDC6 depleted cells are considered defective of DNA repair checkpoint and proceed faster than the control cells in the cell cycle upon induced DNA damage (PMID: 22655027). Specification Tested Reactivity: human, mouse, rat Host / Isotype: Mouse / IgG1 Class: Monoclonal Type: Antibody Immunogen: CatNo: Ag6952 Product name: Recombinant human CCDC6 protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 127-474 aa of BC036757 Sequence: KETLAVNYEKEEEFLTNELSRKLMQLQHEKAELEQHLEQEQEFQVNKLMKKIKKLENDTISKQLTLEQLRREKIDLENTLEQEQEALVNRLWKRMDKLEAEKRILQEKLDQPVSAPPSPRDISMEIDSPENMMRHIRFLKNEVERLKKQLRAAQLQHSEKMAQYLEEERHMREENLRLQRKLQREMERREALCRQLSESESSLEMDDERYFNEMSAQGLRPRTVSSPIPYTPSPSSSRPISPGLSYASHTVGFTPPTSLTRAGMSYYNSPGLHVQHMGTSHGITRPSPRRSNSPDKFKRPTPPPSPNTQTPVQPPPPPPPPPMQPTVPSAATSQPTPSQHSAHTSSQP Predict reactive species Full Name: coiled-coil domain containing 6 Calculated Molecular Weight: 474 aa, 53 kDa Observed Molecular Weight: 65 kDa GenBank Accession Number: BC036757 Gene Symbol: CCDC6 Gene ID (NCBI): 8030 RRID: AB_2882838 Conjugate: Unconjugated Form: Liquid Purification Method: Protein G purification UNIPROT ID: Q16204 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.