Proteintech, 67644-1-Ig, PI3 Kinase p85 Beta Monoclonal antibody

Size: 20ul / 150ul The PI3 Kinase p85 Beta (67644-1-Ig) by Proteintech is a Monoclonal antibody targeting PI3 Kinase p85 Beta in WB, IHC, IF/ICC, ELISA applications with reactivity to Human, Mouse, Rat samples 67644-1-Ig targets PI3 Kinase p85 Beta in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, Mouse, Rat samples. Tested Applications Positive WB detected in: HEK-293 cells, LNCaP cells, HeLa cells, MCF-7 cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells, 4T1 cells, Jurkat cells Positive IHC detected in: human colon tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HeLa cells Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Immunohistochemistry (IHC): IHC : 1:200-1:800 Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600 Background Information PI3 Kinase p85 is also named as PIK3R2 and belongs to the PI3K p85 subunit family. PI3 Kinase p85 is a regulatory subunit of phosphoinositide-3-kinase (PI3K) which is a kinase that phosphorylates phosphatidylinositol 4,5-bisphosphate to generate PIP3. PI3 Kinase p85 binds to activated (phosphorylated) protein-tyrosine kinases through its SH2 domain, and then acts as an adapter to mediate the association of the p110 catalytic unit to the plasma membrane. It promotes nuclear translocation of XBP1 isoform 2 in a ER stress or insulin-dependent manner during metabolic overloading in the liver and hence plays a role in glucose tolerance improvement (PMID:23604317). Specification Tested Reactivity: Human, Mouse, Rat Cited Reactivity: human, mouse, rat Host / Isotype: Mouse / IgG2b Class: Monoclonal Type: Antibody Immunogen: CatNo: Ag14369 Product name: Recombinant human PIK3R2 protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 1-324 aa of BC014170 Sequence: YRHESLAQYNAKLDTRLLYPVSKYQQDQIVKEDSVEAVGAQLKVYHQQYQDKSREYDQLYEEYTRTSQELQMKRTAIEAFNETIKIFEEQGQTQEKCSKEYLERFRREGNEKEMQRILLNSERLKSRIAEIHESRTKLEQQLRAQASDNREIDKRMNSLKPDLMQLRKIRDQYLVWLTQKGARQKKINEWLGIKNETEDQYALMEDEDDLPHHEERTWYVGKINRTQAEEMLSGKRDGTFLIRESSQRGCYACSVVVDGDTKHCVIYRTATGFGFAEPYNLYGSLKELVLHYQHASLVQHNDALTVTLAHPVRAPGPGPPPAAR Predict reactive species Full Name: phosphoinositide-3-kinase, regulatory subunit 2 (beta) Calculated Molecular Weight: 728 aa, 82 kDa Observed Molecular Weight: 85 kDa GenBank Accession Number: BC014170 Gene Symbol: PI3 Kinase p85 Beta Gene ID (NCBI): 5296 RRID: AB_2882844 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: O00459 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.