Proteintech, 67663-1-Ig, IMPDH2 Monoclonal antibody

Size: 20ul / 150ul The IMPDH2 (67663-1-Ig) by Proteintech is a Monoclonal antibody targeting IMPDH2 in WB, IHC, IF/ICC, ELISA applications with reactivity to Human, mouse, rat samples 67663-1-Ig targets IMPDH2 in WB, IHC, IF/ICC, CoIP, ELISA applications and shows reactivity with Human, mouse, rat samples. Tested Applications Positive WB detected in: A549 cells, HSC-T6 cells, 4T1 cells, HeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells, PC-12 cells Positive IHC detected in: human breast hyperplasia tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: A549 cells Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Immunohistochemistry (IHC): IHC : 1:500-1:2000 Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800 Background Information IMPDH2, also named as IMPD2, belongs to the IMPDH/GMPR family. It is a rate limiting enzyme in the de novo synthesis of guanine nucleotides and therefore is involved in the regulation of cell growth. It may also have a role in the development of malignancy and the growth progression of some tumors. IMPDH2 is a key enzyme in the purine nucleotide biosynthetic pathway and constitutes a pivotal biological target for immunosuppressant and antiviral drugs. (PMID:21181270) Specification Tested Reactivity: Human, mouse, rat Cited Reactivity: human Host / Isotype: Mouse / IgG1 Class: Monoclonal Type: Antibody Immunogen: CatNo: Ag17170 Product name: Recombinant human IMPDH2 protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 1-350 aa of BC012840 Sequence: MADYLISGGTSYVPDDGLTAQQLFNCGDGLTYNDFLILPGYIDFTADQVDLTSALTKKITLKTPLVSSPMDTVTEAGMAIAMALTGGIGFIHHNCTPEFQANEVRKVKKYEQGFITDPVVLSPKDRVRDVFEAKARHGFCGIPITDTGRMGSRLVGIISSRDIDFLKEEEHDCFLEEIMTKREDLVVAPAGITLKEANEILQRSKKGKLPIVNEDDELVAIIARTDLKKNRDYPLASKDAKKQLLCGAAIGTHEDDKYRLDLLAQAGVDVVVLDSSQGNSIFQINMIKYIKDKYPNLQVIGGNVVTAAQAKNLIDAGVDALRVGMGSGSICITQEVLACGRPQATAVYKV Predict reactive species Full Name: IMP (inosine monophosphate) dehydrogenase 2 Calculated Molecular Weight: 514 aa, 56 kDa Observed Molecular Weight: 56 kDa GenBank Accession Number: BC012840 Gene Symbol: IMPDH2 Gene ID (NCBI): 3615 RRID: AB_2882860 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: P12268 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.