Proteintech, 67683-1-Ig, RPS12 Monoclonal antibody

Size: 20ul / 150ul The RPS12 (67683-1-Ig) by Proteintech is a Monoclonal antibody targeting RPS12 in WB, IHC, IF/ICC, ELISA applications with reactivity to human, mouse, rat samples 67683-1-Ig targets RPS12 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: HeLa cells, HepG2 cells, NCI-H1299 cells, HEK-293 cells, HSC-T6 cells, NIH/3T3 cells, PC-12cells, 4T1 cells Positive IHC detected in: human breast cancer tissue, human colon cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HepG2 cells Recommended dilution Western Blot (WB): WB : 1:2000-1:10000 Immunohistochemistry (IHC): IHC : 1:250-1:1000 Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500 Background Information RPS12 (ribosomal protein S12) is a plastid ribosomal protein which is a part of the 30S ribosomal subunit. Together with S4 and S5 plays an important role in translational accuracy. Specification Tested Reactivity: human, mouse, rat Host / Isotype: Mouse / IgG1 Class: Monoclonal Type: Antibody Immunogen: CatNo: Ag9649 Product name: Recombinant human RPS12 protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 1-132 aa of BC017321 Sequence: MAEEGIAAGGVMDVNTALQEVLKTALIHDGLARGIREAAKALDKRQAHLCVLASNCDEPMYVKLVEALCAEHQINLIKVDDNKKLGEWVGLCKIDREGKPRKVVGCSCVVVKDYGKESQAKDVIEEYFKCKK Predict reactive species Full Name: ribosomal protein S12 Calculated Molecular Weight: 132 aa, 15 kDa Observed Molecular Weight: 15 kDa GenBank Accession Number: BC017321 Gene Symbol: RPS12 Gene ID (NCBI): 6206 RRID: AB_2882876 Conjugate: Unconjugated Form: Liquid Purification Method: Protein G purification UNIPROT ID: P25398 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.