Proteintech, 67775-1-Ig, ANKRD1 Monoclonal antibody

Size: 20ul / 150ul The ANKRD1 (67775-1-Ig) by Proteintech is a Monoclonal antibody targeting ANKRD1 in WB, IF/ICC, ELISA applications with reactivity to human, mouse, rat, pig, rabbit samples 67775-1-Ig targets ANKRD1 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit samples. Tested Applications Positive WB detected in: mouse heart tissue, rabbit heart tissue, pig heart tissue Positive IF/ICC detected in: A431 cells Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Immunofluorescence (IF)/ICC: IF/ICC : 1:1000-1:4000 Background Information Ankyrin repeat domain-containing protein 1(ANKRD1) belongs to the conserved muscle ankyrin repeat protein(MARP) family, whose expression is induced in response to physiologic stress, injury and hypertrophy. Thus its expression mainly in activated vascular endothelial cells. ANKRD1 may regulate SMC proliferation through the CDKN1A pathway. It functions as a nuclear transcription repressor that regulate cardiac gene's expression Specification Tested Reactivity: human, mouse, rat, pig, rabbit Cited Reactivity: human, mouse Host / Isotype: Mouse / IgG2b Class: Monoclonal Type: Antibody Immunogen: CatNo: Ag29974 Product name: Recombinant human ANKRD1 protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 1-319 aa of BC018667 Sequence: MMVLKVEELVTGKKNGNGEAGEFLPEDFRDGEYEAAVTLEKQEDLKTLLAHPVTLGEQQWKSEKQREAELKKKKLEQRSKLENLEDLEIIIQLKKRKKYRKTKVPVVKEPEPEIITEPVDVPTFLKAALENKLPVVEKFLSDKNNPDVCDEYKRTALHRACLEGHLAIVEKLMEAGAQIEFRDMLESTAIHWASRGGNLDVLKLLLNKGAKISARDKLLSTALHVAVRTGHYECAEHLIACEADLNAKDREGDTPLHDAVRLNRYKMIRLLIMYGADLNIKNCAGKTPMDLVLHWQNGTKAIFDSLRENSYKTSRIATF Predict reactive species Full Name: ankyrin repeat domain 1 (cardiac muscle) Calculated Molecular Weight: 319 aa, 36 kDa GenBank Accession Number: BC018667 Gene Symbol: ANKRD1 Gene ID (NCBI): 27063 RRID: AB_2918540 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: Q15327 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.