Proteintech, 67778-1-Ig, Phospho-mTOR (Ser2448) Monoclonal antibody

Size: 20ul / 100ul The Phospho-mTOR (Ser2448) (67778-1-Ig) by Proteintech is a Monoclonal antibody targeting Phospho-mTOR (Ser2448) in WB, IF/ICC, FC (Intra), ELISA applications with reactivity to human, mouse, rat samples 67778-1-Ig targets Phospho-mTOR (Ser2448) in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: HeLa cells, NIH/3T3 cells, HEK-293T cells, HEK-293 cells, HSC-T6 cells, Calyculin A treated HeLa cells, EGF treated NIH/3T3 cells, Rapamycin treated HEK-293 cells, Calyculin A treated HEK-293 cells Positive IF/ICC detected in: HepG2 cells Positive FC (Intra) detected in: Calyculin A treated HeLa cells, 100 nM Calyculin A (30 minutes) treated HeLa cells Recommended dilution Western Blot (WB): WB : 1:2000-1:10000 Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500 Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.50 ug per 10^6 cells in a 100 µl suspension Background Information MTOR, also named as FRAP1, FRAP, FRAP2 and RAPT1, belongs to the PI3/PI4-kinase family. MTOR is a Ser/Thr protein kinase that functions as an ATP and amino acid sensor to balance nutrient availability and cell growth. MTOR is kinase subunit of both mTORC1 and mTORC2, which regulate cell growth and survival in response to nutrient and hormonal signals. mTORC1 is activated in response to growth factors or amino-acids. mTORC2 is also activated by growth factors, but seems to be nutrient-insensitive. mTORC2 seems to function upstream of Rho GTPases to regulate the actin cytoskeleton, probably by activating one or more Rho-type guanine nucleotide exchange factors. mTORC2 promotes the serum-induced formation of stress-fibers or F-actin. mTOR is phosphorylated at Ser2448 via the PI3 kinase/Akt signaling pathway and autophosphorylated at Ser2481. mTOR plays a key role in cell growth and homeostasis and may be abnormally regulated in tumors. Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human, mouse, rat, pig, chicken, bovine Host / Isotype: Mouse / IgG2b Class: Monoclonal Type: Antibody Immunogen: Peptide Predict reactive species Full Name: FK506 binding protein 12-rapamycin associated protein 1 Calculated Molecular Weight: 289 kDa Observed Molecular Weight: 289 kDa GenBank Accession Number: BC117166 Gene Symbol: mTOR Gene ID (NCBI): 2475 RRID: AB_2889842 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: P42345 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.