Proteintech, 67792-1-Ig, SERBP1 Monoclonal antibody

Size: 20ul / 150ul The SERBP1 (67792-1-Ig) by Proteintech is a Monoclonal antibody targeting SERBP1 in WB, IHC, IF-P, IP, ELISA applications with reactivity to Human, Mouse, Rat, pig, Canine samples 67792-1-Ig targets SERBP1 in WB, IHC, IF-P, IP, ELISA applications and shows reactivity with Human, Mouse, Rat, pig, Canine samples. Tested Applications Positive WB detected in: LNCaP cells, MDCK cells, HSC-T6 cells, HeLa cells, HEK-293 cells, Jurkat cells, K-562 cells Positive IP detected in: HeLa cells Positive IHC detected in: human liver cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF-P detected in: human liver cancer tissue Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:500-1:2000 Immunofluorescence (IF)-P: IF-P : 1:200-1:800 Background Information The plasminogen activator system (PA) plays an important role in invasion and metastasis of solid tumors. The PA Inhibitor type 1 (PAI-1) is the main physiologic regulator of plasminogen activation. PAI-RBP1 (PAI-1 mRNA Binding Protein 1, also named SERBP1), is an RNA-binding protein containing an RG-rich domain and an RGG box domain that might be methylated by protein arginine N-methyltransferases (PRMTs). SERBP1 was identified as an interacting partner of a Tudor domain containing protein TDRD3, and can localize to cytoplasmic stress granules (SGs). Specification Tested Reactivity: Human, Mouse, Rat, pig, Canine Host / Isotype: Mouse / IgG1 Class: Monoclonal Type: Antibody Immunogen: CatNo: Ag30202 Product name: Recombinant human SERBP1 protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 108-408 aa of BC008045 Sequence: RVGRRPDQQLQGEGKIIDRRPERRPPRERRFEKPLEEKGEGGEFSVDRPIIDRPIRGRGGLGRGRGGRGRGMGRGDGFDSRGKREFDRHSGSDRSSFSHYSGLKHEDKRGGSGSHNWGTVKDELTESPKYIQKQISYNYSDLDQSNVTEETPEGEEHHPVADTENKENEVEEVKEEGPKEMTLDEWKAIQNKDRAKVEFNIRKPNEGADGQWKKGFVLHKSKSEEAHAEDSVMDHHFRKPANDITSQLEINFGDLGRPGRGGRGGRGGRGRGGRPNRGSRTDKSSASAPDVDDPEAFPALA Predict reactive species Full Name: SERPINE1 mRNA binding protein 1 Calculated Molecular Weight: 45 kDa Observed Molecular Weight: 50 kDa GenBank Accession Number: BC008045 Gene Symbol: SERBP1 Gene ID (NCBI): 26135 RRID: AB_2918556 Conjugate: Unconjugated Form: Liquid Purification Method: Protein G purification UNIPROT ID: Q8NC51 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.