Proteintech, 67830-1-Ig, BAD Monoclonal antibody

Size: 20ul / 150ul BAD Monoclonal Antibody for WB, IF/ICC, ELISA 67830-1-Ig targets BAD in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: LNCaP cells, RAW 264.7 cells, HeLa cells, HEK-293 cells, Jurkat cells, Raji cells, THP-1 cells, HSC-T6 cells Positive IF/ICC detected in: HeLa cells Recommended dilution Western Blot (WB): WB : 1:1000-1:6000 Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800 Background Information BAD, also named as BBC6 and BCL2L8, promotes cell death. It is successfully competes for the binding to Bcl-X(L), Bcl-2 and Bcl-W, thereby affecting the level of heterodimerization of these proteins with BAX. BAD can reverse the death repressor activity of Bcl-X(L), but not that of Bcl-2. It appears to act as a link between growth factor receptor signaling and the apoptotic pathways. Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human, mouse, rat Host / Isotype: Mouse / IgG1 Class: Monoclonal Type: Antibody Immunogen: Peptide Predict reactive species Full Name: BCL2-associated agonist of cell death Calculated Molecular Weight: 18 kDa Observed Molecular Weight: 18-25 kDa GenBank Accession Number: BC001901 Gene Symbol: BAD Gene ID (NCBI): 572 RRID: AB_2918592 Conjugate: Unconjugated Form: Liquid Purification Method: Protein G purification UNIPROT ID: Q92934 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.