Proteintech, 67936-1-Ig, SETD1A Monoclonal antibody

Size: 20ul / 150ul The SETD1A (67936-1-Ig) by Proteintech is a Monoclonal antibody targeting SETD1A in WB, ELISA applications with reactivity to Human, mouse, rat samples 67936-1-Ig targets SETD1A in WB, IF, ELISA applications and shows reactivity with Human, mouse, rat samples. Tested Applications Positive WB detected in: HeLa cells, LNCaP cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells, mouse brain tissue, HEK-293 cells, HepG2 cells, Jurkat cells Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Background Information SETD1A is a SET domain-containing methyltransferase involved in the epigenetic regulation of transcription(PMID:12670868). It is the main catalytic component of a multiprotein complex that methylates lysine 4 of histone H3, a histone mark associated with gene activation (PMID:29937342). Responsible for H3K4me3 enriched promoters and transcriptional programming of inner mass stem cells and neuron progenitors during embryogenesis (PMID:31197650). Does not methylate 'Lys-4' of histone H3 if the neighboring 'Lys-9' residue is already methylated (PubMed:12670868). SETD1A has a calculated molecular weight of 186 kDa, and a 258 kDa band was also reported (PMID: 31076518). Specification Tested Reactivity: Human, mouse, rat Cited Reactivity: human, mouse Host / Isotype: Mouse / IgG1 Class: Monoclonal Type: Antibody Immunogen: CatNo: Ag30424 Product name: Recombinant human SETD1A protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-160 aa of NM_014712 Sequence: MDQEGGGDGQKAPSFQWRNYKLIVDPALDPALRRPSQKVYRYDGVHFSVNDSKYIPVEDLQDPRCHVRSKNRDFSLPVPKFKLDEFYIGQIPLKEVTFARLNDNVRETFLKDMCRKYGEVEEVEILLHPRTRKHLGLARVLFTSTRGAKETVKNLHLTSV Predict reactive species Full Name: SET domain containing 1A Calculated Molecular Weight: 186 kDa Observed Molecular Weight: 270 kDa GenBank Accession Number: NM_014712 Gene Symbol: SETD1A Gene ID (NCBI): 9739 RRID: AB_2918688 Conjugate: Unconjugated Form: Liquid Purification Method: Protein G purification UNIPROT ID: O15047 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.