Proteintech, 67968-1-Ig, Flotillin 1 Monoclonal antibody

Size: 20ul / 150ul The Flotillin 1 (67968-1-Ig) by Proteintech is a Monoclonal antibody targeting Flotillin 1 in WB, FC (Intra), ELISA applications with reactivity to human, mouse, rat, pig, rabbit samples 67968-1-Ig targets Flotillin 1 in WB, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit samples. Tested Applications Positive WB detected in: LNCaP cells, U-251 cells, Raji cells, Jurkat cells, MOLT-4 cells, human peripheral blood platelets, pig brain tissue, rabbit brain tissue, rat brain tissue, mouse brain tissue Positive FC (Intra) detected in: Raji cells Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension Background Information Flotillins are membrane-associated proteins that contain two homologous isoforms, flotillin 1 (FLOT1) and flotillin 2 (FLOT2). They are highly conserved and widely expressed proteins that commonly used as markers of lipid rafts. Flotillins are involved in various signaling pathways, cell adhesion, membrane trafficking and axonal growth. Specification Tested Reactivity: human, mouse, rat, pig, rabbit Host / Isotype: Mouse / IgG2b Class: Monoclonal Type: Antibody Immunogen: CatNo: Ag8694 Product name: Recombinant human Flotillin 1 protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 80-427 aa of BC001146 Sequence: MLAAACQMFLGKTEAEIAHIALETLEGHQRAIMAHMTVEEIYKDRQKFSEQVFKVASSDLVNMGISVVSYTLKDIHDDQDYLHSLGKARTAQVQKDARIGEAEAKRDAGIREAKAKQEKVSAQYLSEIEMAKAQRDYELKKAAYDIEVNTRRAQADLAYQLQVAKTKQQIEEQRVQVQVVERAQQVAVQEQEIARREKELEARVRKPAEAERYKLERLAEAEKSQLIMQAEAEAASVRMRGEAEAFAIGARARAEAEQMAKKAEAFQLYQEAAQLDMLLEKLPQVAEEISGPLTSANKITLVSSGSGTMGAAKVTGEVLDILTRLPESVERLTGVSISQVNHKPLRTA Predict reactive species Full Name: flotillin 1 Calculated Molecular Weight: 47 kDa Observed Molecular Weight: 47 kDa GenBank Accession Number: BC001146 Gene Symbol: Flotillin 1 Gene ID (NCBI): 10211 RRID: AB_2918719 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: O75955 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.