Proteintech, 67981-1-Ig, PRMT6 Monoclonal antibody

Size: 20ul / 150ul The PRMT6 (67981-1-Ig) by Proteintech is a Monoclonal antibody targeting PRMT6 in WB, ELISA applications with reactivity to human, mouse, rat samples 67981-1-Ig targets PRMT6 in WB, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: HeLa cells, MCF-7 cells, HEK-293 cells, HepG2 cells, Jurkat cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Background Information Protein arginine methyltransferase 6 (PRMT6) is a type I PRMT which is involved in epigenetic regulation of gene expression, alternative splicing, development and differentiation, DNA repair, cell proliferation and senescence, DNA methylation, mitosis, inflammation, innate antiviral immunity, spermatogenesis, transactivation of nuclear receptors and cell signaling. The human PRMT6 gene, located on Chromosome 1, encodes for the 41.9 kDa PRMT6 enzyme. PRMT6 is predominantly localized to the nucleus, in stark contrast to PRMT3 and PRMT5 which are preponderantly cytosolic, while other PRMTs are found in both nucleus and cytosol. PRMT6 is expressed in a wide range of tissues with high expression in kidney and testes. PRMT6 generates asymmetric dimethylation modifications in histone 3 at arginine 2, arginine 17 and arginine 42 (H3R2me2a, H3R17me2a and H3R42me2a) and in histone H2A at arginine 26 (H2AR26me2a) and participates in the epigenetic regulation of gene expression. Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human Host / Isotype: Mouse / IgG2a Class: Monoclonal Type: Antibody Immunogen: CatNo: Ag7934 Product name: Recombinant human PRMT6 protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 1-316 aa of BC002729 Sequence: MIADRVRTDAYRLGILRNWAALRGKTVLDVGAGTGILSIFCAQAGARRVYAVEASAIWQQAREVVRFNGLEDRVHVLPGPVETVELPEQVDAIVSEWMGYGLLHESMLSSVLHARTKWLKEGGLLLPASAELFIAPISDQMLEWRLGFWSQVKQHYGVDMSCLEGFATRCLMGHSEIVVQGLSGEDVLARPQRFAQLELSRAGLEQELEAGVGGRFRCSCYGSAPMHGFAIWFQVTFPGGESEKPLVLSTSPFHPATHWKQALLYLNEPVQVEQDTDVSGEITLLPSRDNPRRLRVLLRYKVGDQEEKTKDFAMED Predict reactive species Full Name: protein arginine methyltransferase 6 Calculated Molecular Weight: 42 kDa Observed Molecular Weight: 42 kDa GenBank Accession Number: BC002729 Gene Symbol: PRMT6 Gene ID (NCBI): 55170 RRID: AB_2918730 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: Q96LA8 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.