Proteintech, 68020-1-Ig, Angiotensinogen/AGT Monoclonal antibody

Size: 20ul / 150ul The Angiotensinogen/AGT (68020-1-Ig) by Proteintech is a Monoclonal antibody targeting Angiotensinogen/AGT in WB, IHC, IF/ICC, ELISA applications with reactivity to human, pig samples 68020-1-Ig targets Angiotensinogen/AGT in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, pig samples. Tested Applications Positive WB detected in: human plasma, HuH-7 cells, THP-1 cells, human placenta tissue, pig plasma Positive IHC detected in: human kidney tissue, human liver tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HepG2 cells Recommended dilution Western Blot (WB): WB : 1:5000-1:20000 Immunohistochemistry (IHC): IHC : 1:500-1:2000 Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800 Background Information Angiotensinogen is a precursor of angiotensin II (Ang II), is expressed and synthesized largely in the liver and is cleaved by the enzyme renin in response to lowered blood pressure. It has a key role in mediating vascular constriction and regulating salt and fluid homeostasis. The resulting product, angiotensin I, is then cleaved by angiotensin converting enzyme (ACE) to generate the physiologically active enzyme angiotensin II. Mutations in this gene are associated with susceptibility to essential hypertension, and can cause renal tubular dysgenesis, a severe disorder of renal tubular development. Defects in this gene also have been associated with non-familial structural atrial fibrillation, and inflammatory bowel disease. Specification Tested Reactivity: human, pig Cited Reactivity: pig Host / Isotype: Mouse / IgG2b Class: Monoclonal Type: Antibody Immunogen: CatNo: Ag16658 Product name: Recombinant human AGT protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 136-485 aa of BC011519 Sequence: PTAVFGTLASLYLGALDHTADRLQAILGVPWKDKNCTSRLDAHKVLSALQAVQGLLVAQGRADSQAQLLLSTVVGVFTAPGLHLKQPFVQGLALYTPVVLPRSLDFTELDVAAEKIDRFMQAVTGWKTGCSLTGASVDSTLAFNTYVHFQGKMKGFSLLAEPQEFWVDNSTSVSVPMLSGMGTFQHWSDIQDNFSVTQVSFTESACLLLIQPHYASDLDKVEGLTFQQNSLNWMKKLSPRTIHLTMPQLVLQGSYDLQDLLAQAELPAILHTELNLQKLSNDRIRVGEVLNSIFFELEADEREPTESTQQLNKPEVLEVTLNRPFLFAVYDQSATALHFLGRVANPLSTA Predict reactive species Full Name: angiotensinogen (serpin peptidase inhibitor, clade A, member 8) Calculated Molecular Weight: 485 aa, 53 kDa Observed Molecular Weight: 52 kDa GenBank Accession Number: BC011519 Gene Symbol: AGT Gene ID (NCBI): 183 RRID: AB_2918765 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: P01019 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.